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Updated: May 16, 2025

Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry
13:06

Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry

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开发一种基于基化的检测方法,用于通过双重MALDI-TOF质谱测量来检测oligo/polysialic acid结构

Kai Suzuki1, Di Wu1,2, Ken Kitajima2

  • 1Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601, Japan.

BBA advances
|April 1, 2025
PubMed
概括

我们开发了一种可甲基化方法,用于改进含有甘氨酸的酸 (Sia) 的质谱分析. 这种技术使得第一个成功检测和测序的多酸 (polySia) 结构,包括那些与O-链接的甘氨酸.

关键词:
马尔迪TOF/TOF/马尔迪土耳其土耳其土耳其质谱测量质量谱测量橄酸酸是酸中的一种.通过甲基化进行了甲基化.聚酸是什么? 聚酸是什么?聚合糖蛋白是一种多合糖蛋白.

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