工程益生菌减轻由纳米塑料诱导的肠道屏障功能障碍
Wenxin Chen1, Qiyan Guo1, Hong Li1
1School of Life and Health Sciences, Hainan Province Key Laboratory of One Health, Collaborative Innovation Center of One Health, Hainan University, Haikou, 570228, China.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|April 1, 2025
概括
这项研究开发了一种益生菌药物输送系统 (EcNT@L) 来对抗纳米塑料 (PET) 对肠道屏障造成的损伤. 该系统有效地减少了炎症和感染,为纳米塑料诱导的肠道功能障碍提供了一种新的治疗方法.
科学领域:
- 环境科学与健康 环境科学与健康
- 微生物学 微生物学
- 生物技术是生物技术.
背景情况:
- 微塑料和纳米塑料,特别是聚乙烯二甲 (PET),损害了肠道屏障的完整性,增加了对炎症和感染的易感性.
- 对于纳米塑料引起的肠道问题的现有治疗方法是有限的,需要创新的解决方案.
研究的目的:
- 开发一种基于益生菌的药物输送系统 (EcNT@L),以减轻纳米塑料诱导的肠道屏障功能障碍.
- 评估EcNT@L在预防和治疗胃肠道炎症和感染方面的疗效.
主要方法:
- 基因工程Escherichia coli Nissle 1917 (EcN) 产生转化生长因子-β (TGF-β) 并涂上Eudragit L100-55以创建EcNT@L.
- 通过NF-κB通路激活评估了EcNT@L对Caco-2细胞对纳米PET诱导的炎症和感染的保护作用.
- 在鼠标模型中评估了EcNT@L在体内治疗疗效的胃肠道感染与纳米PET和沙门氏菌相结合.
主要成果:
- EcNT@L通过激活NF-κB信号通路,有效地保护Caco-2细胞免受纳米PET诱导的炎症和感染.
- 在体内研究表明,EcNT@L在治疗联合纳米PET和沙门氏菌感染方面表现优于商业抗生素.
- EcNT@L调节了免疫反应和肠道微生物群,证明了卓越的治疗效果.
结论:
- 基于益生菌的药物输送系统,如EcNT@L,显示出解决纳米塑料诱导的肠道功能障碍的巨大潜力.
- EcNT@L提供了一种有希望的策略,以减轻微型和纳米塑料对胃肠道系统的健康影响.
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