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一个智能单环介导的同热放大方便了灵活的SNP探头设计,用于现场快速区分SARS-CoV-2Omicron变种

Qijie Lin¹, Hongchao Gou², Xiaoyun Qu¹

¹National and Regional Joint Engineering Laboratory for Medicament of Zoonoses Prevention and Control, Key Laboratory of Zoonoses, Ministry of Agriculture, Key Laboratory of Zoonoses Prevention and Control of Guangdong Province, Animal Infectious Diseases Laboratory, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

|April 1, 2025

概括

一种新的智能单环介导同热放大 (ssLAMP) 方法使用独特的原料设计快速识别SARS-CoV-2 Omicron变体. 这种临床检测为变种监测提供了高灵敏度和特异性.

科学领域:

分子生物学分子生物学
病毒学病毒学
生物技术是生物技术.

背景情况:

有效监测SARS-CoV-2变种的关注需要快速,现场打字方法.
区分Omicron亚型 (BA.1,BA.2,BA.3,BA.4,BA.5) 对于公共卫生反应至关重要.

研究的目的:

开发一种智能单环介导同热放大 (ssLAMP) 方法,用于快速区分SARS-CoV-2 Omicron变种.
创建一个便携式的,点的护理系统实时变种检测.

主要方法:

设计了一种新的ssLAMP测定方法,省略了内部原料,并结合了单核酸多态 (SNP) 识别的群聚原料.
一个3D打印的便携式双光可视化设备与智能手机应用程序相结合,被开发用于样本分析.
测试的灵敏度,特异性和速度使用合成标和临床鼻抽样进行了评估.

主要成果:

该ssLAMP方法通过SNP识别证明了高灵敏度 (100拷贝/反应) 和特异性.
该试验成功地从97个临床标本中识别了5个BA.5-阳性样本,与桑格测序100%一致.
整个检测过程,包括样本分析,在90分钟内完成.

结论:

开发的ssLAMP试验提供了一种快速,灵敏和特定的方法,用于现场检测和区分SARS-CoV-2 Omicron变种.
便携式护理点系统有助于实时监测,并有潜在的应用在其他领域的病原体基因定型和SNP突变检测.

关键词:

令人担忧的SARS-CoV-2变种在SNP检测检测.核酸放大核酸放大 ssLAMPP 这是一灯.

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Single Nucleotide Polymorphisms-SNPs

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A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...