菌染色体重塑剂Isw1通过调节tRNA转录来调节翻译
Jing Wang1,2, Yueqi Zhang2, Jingrui Wang2
1Department of Plant Pathology, College of Plant Protection, Southwest University, Chongqing 400715, China.
Nucleic acids research
|April 1, 2025
概括
染色体重塑剂FgIsw1通过维持转移RNA (tRNA) 转录,影响蛋白质转化和菌毒素生产来调节真菌毒性至关重要.
科学领域:
- 分子生物学分子生物学
- 菌类学 菌类学是指菌类学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 染色体动态调节DNA过程,并受到环境因素的影响.
- ISWI家族酶是已知的染色体重塑剂,但它们的真核细胞功能在很大程度上是未知的.
- 普特雷辛诱导了Fusarium graminearum中脱氧烯醇 (DON) 生物合成,改变了色素的可访问性.
研究的目的:
- 研究Fusarium graminearum中依赖ATP的染色质重塑剂FgIsw1的作用.
- 确定FgIsw1对色素可访问性和对普特雷辛的反应中的基因转录的影响.
- 阐明FgIsw1调节DON生物合成和真菌毒性的机制.
主要方法:
- 在Fusarium graminearum中分析染色质可访问性,使用和不使用putrescine.
- 基因删除研究以评估FgIsw1的功能.
- 转移RNA (tRNA) 转录和DON生物合成基因表达的测量.
- 对宿主植物的真菌毒性的评估.
主要成果:
- 普特雷辛在全球范围内改变了染色质的可访问性,FgIsw1作为关键调节剂.
- 删除FgIsw1破坏了tRNA转录,但没有直接破坏DON生物合成基因.
- FgIsw1在tRNA染色体区域中保持核细胞相位,确保有效的tRNA转录.
- ΔFgIsw1菌株显示了DON生物合成酶的翻译减少,缺乏DON生产,并失去毒性.
结论:
- 通过控制tRNA转录,FgIsw1在调节蛋白质翻译方面发挥了新的作用.
- 染色体重塑对于通过tRNA调节维持真菌毒性至关重要.
- FgIsw1是一个关键因素,将染色质动态与菌毒素产生和宿主-病原体相互作用联系起来.
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