相关实验视频

Updated: May 16, 2025

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Published on: March 11, 2020

基亚萨努尔森林疾病的分子流行病学使用ONT-NGS,一个前进测序的领域

Shashi Sharma¹, Pooja Yadav¹, Paban Kumar Dash¹

¹Virology Division, Defence Research Development Establishment, Jhansi Road, Gwalior 474002, India.

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology

|April 1, 2025

概括

从2018年到2020年对Kyasanur森林疾病病毒 (KFDV) 的基因组测序揭示了新的循环菌株. 这项研究为印度KFDV监测和疫苗开发提供了关键的分子数据.

科学领域:

* 病毒学病毒学
* 分子流行病学
* 生物信息学是一门学科.

背景情况:

*凯萨努尔森林病 (KFD) 是印度的一个重大公共卫生问题,其特点是季节性爆发.
* 关于最近流传的Kyasanur森林疾病病毒 (KFDV) 菌株的基因组信息存在关键知识差距.
* 实地前进,现场策略对于及时检测传染病原体和分子表征至关重要.

研究的目的:

* 通过现场测序,破译最近流传的Kyasanur森林疾病病毒 (KFDV) 菌株的整个基因组.
* 分析2018-2020年间的针对KFDV监控数据.
* 提供基因组见解,以改善针对KFDV的诊断和疫苗策略.

主要方法:

* 2018-2020年使用牛津纳米孔技术 (ONT) 下一代测序 (NGS) 测序Kyasanur森林疾病病毒 (KFDV) PCR阳性样本的全基因组测序.
*对测序的KFDV基因组进行了详细的分子遗传学分析.
* 在流通的KFDV菌株中检测和分析非同名变异.

主要成果:

* 19个Kyasanur森林疾病病毒 (KFDV) 的整个基因组从印度卡纳塔克邦的Shivamogga地区收集的样本中成功测序.
*在2018年至2020年期间流传的KFDV菌株中,发现了28种非同名变种.
*在80%的样本中观察到一种占主导地位的变异,病毒包膜蛋白中有超过10个变化,这表明最近的占主导地位的血统.

结论:

*本研究是首次报告使用现场前进方法研究最近流传的Kyasanur森林疾病病毒 (KFDV) 菌株.
*生成的基因组数据为了解KFDV演变和流行病学提供了有价值的信息.
*这些研究结果将有助于开发和完善诊断工具和疫苗策略,以对抗山森林疾病.

关键词:

在KFD中,KFD就是KFD.这是一个纳米孔.在线播放在线播放 ONT ONT 序列化是指测序的使用.

更多相关视频

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Published on: April 4, 2018

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings

Published on: August 18, 2023

相关概念视频

Single Nucleotide Polymorphisms-SNPs

Single Nucleotide Polymorphisms-SNPs

A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...

Evolutionary Relationships through Genome Comparisons

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...