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一个染色体水平的单双型解析基因组组合和注释的皮塔亚 (Selenicereus polyrhizus)

Juncheng Li¹, Wenlong Luo², Biao Jiang²

¹Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences; Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture and Rural Affairs; Guangdong Provincial Key Laboratory of Science and Technology Research on Fruit Tree, Guangzhou, 510640, China.

Scientific data

|April 1, 2025

概括

我们向您介绍了第一个染色体级,哈普洛型解析的皮塔亚 (Selenicereus spp.) 的基因组组合. 这种高质量的皮塔亚基因组资源将促进其生态,进化和繁殖方面的研究.

科学领域:

基因组学就是基因组学.
植物生物学植物生物学
生物信息学是一种生物信息学.

背景情况:

皮塔亚 (Selenicereus spp.) 是一种植物. 是一种具有经济意义的水果作物,起源于北美和中美洲.
中国和越南的广泛种植凸显了它的全球农业意义.
对皮塔亚的遗传构成有全面的了解,对于作物改进和研究至关重要.

研究的目的:

为了生成一个高质量的,染色体水平,哈普洛型解析的基因组组合 pitaya.
为皮塔亚研究提供基础基因组资源.
为了促进未来研究皮塔亚的进化,生态和繁殖.

主要方法:

使用了PacBio HiFi和牛津纳米孔超长测序技术.
使用Hi-C数据来帮助基因组组装.
生物信息分析用于基因预测和注释.

主要成果:

组装了两个不同的单元型基因组 (hap1: 1.477 Gb,hap2: 1.442 Gb).
超过96%的每种单个类型基因组定在11个伪染色体上.
该组合预测了成千上万的蛋白质编码基因和非编码RNA,注释率高.

结论:

这项研究成功地产生了一种高质量的,平型解析的皮塔亚基因组.
这种基因组资源对于推进皮塔亚的生态,进化,生物和育种研究来说是无价的.
详细的基因组信息将支持未来的遗传和作物改进计划.

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Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.