双向转录因子BAI1在陆地植物中协调核和塑基因转录
Jing Qin1, Yelin Shan2, Hao Liu1
1Chinese Education Ministry's Key Laboratory of Western Resources and Modern Biotechnology, Key Laboratory of Biotechnology Shaanxi Province, College of Life Sciences, Northwest University, Xi'an 710069, China.
Molecular plant
|April 2, 2025
概括
BAI1是一种双向的转录因子,通过调节核和塑基因转录,对叶绿体生物发生至关重要. 它的损失导致白化种子,突出其在协调光合作用和植物生存中的作用.
科学领域:
- 分子生物学分子生物学
- 植物科学 植物科学
- 遗传学 是一个遗传学.
背景情况:
- 塑体和细胞核之间的协调转录对于在质细胞生物发生过程中组装光合作用装置至关重要.
- 虽然已知光合作用的核转录因子,但调节塑性质基因转录的核转录因子在很大程度上没有表征.
研究的目的:
- 识别和描述参与塑基因调节和叶绿体生物发生的新型转录因子.
- 为了阐明BAI1协调核和塑基因表达的机制,用于Rubisco组装.
主要方法:
- 阿拉比多普西斯BAI1 (阿尔比诺) 突变的特征,这是一个核-塑双向的C2H2型指转录因子.
- 分析BAI1与塑性转录活性染色体 (pTAC) 综合体的相互作用及其在RNA聚合酶组合中的作用.
- 在bai1突变体中对PEP和NEP依赖基因的基因表达分析.
- 电泳运动转移试验 (EMSAs) 证实BAI1直接结合到向基因促进体.
- 使用其他植物物种同源BAI1基因的补充研究.
主要成果:
- 阿拉比多普西斯BAI1的淘汰导致白化种子和致命性,证实了它在质细胞形成中的重要作用.
- BAI1 作为 pTAC 复合物的组成部分,与 pTAC12/PAP5/HMR 相互作用,以促进塑性质编码RNA聚合酶 (PEP) 复合物的组合.
- 该bai1突变体表现出PEP依赖基因的转录水平降低和NEP依赖转录的增加,表明BAI1在维持PEP活动中的作用.
- BAI1直接与核RbcS和塑RbcL基因的促进子区域结合,激活它们的Rubisco组装表达.
- 来自各种植物物种的BAI1同类物质可以补充Arabidopsis bai1突变物,表明功能得到保护.
结论:
- BAI1是一种关键的核-塑双标转录因子,它同步核和塑基因表达,用于质细胞生物发生和Rubisco组装.
- 在pTAC复合体内BAI1的功能突出显示了塑基因转录和PEP活动的新型调节机制.
- BAI1和HMR的保存进化路径表明,它们的共同进化通过高效的光合作用促进了植物的地球化.
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