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维生素B₆辅因子衍生AIEE活性光原用于检测西普罗夫洛克萨和莱沃弗洛克萨

Dhvani A Patel¹, Bigyan R Jali², Suban K Sahoo¹

¹Department of Chemistry, Sardar Vallabhbhai National Institute of Technology, Surat, Gujarat, India.

Luminescence : the journal of biological and chemical luminescence

|April 2, 2025

概括

一种来自维生素B6的新型光原体表现出聚合诱导的排放增强,可使人体血清中敏感检测出西普罗素和利沃素.

科学领域:

材料科学材料科学材料科学
分析化学分析化学
生物化学生物化学

背景情况:

聚合诱导的排放增强 (AIEE) 是一种有价值的光物理现象.
维生素B6衍生物为新型光原体的开发提供了潜力.
开发药品敏感检测方法至关重要.

研究的目的:

从维生素B6中合成和表征一种新的AIEE光原体 (AIEEgen).
为了研究合成的NPL的AIEE特性.
探索NPL在生物样本中检测诺基诺抗生素的潜力.

主要方法:

通过氧化5'-酸盐 (PLP) 和3-基-2-纳夫托化的凝结合成NPL.
在DMSO-HEPES缓冲器中描述AIEE属性.
使用光光谱学,UV-Vis吸收,DLS,泽塔潜力和SEM分析药物-NPL相互作用.
人类血清中西普罗夫洛克萨和莱沃弗洛克萨的量化.

主要成果:

NPL表现出AIEE的特征,在聚合时将排放从黄色转变为蓝色.
齐普罗夫洛克萨和莱沃弗洛克萨的存在显著增强了NPL光.
证实了NPL和药物之间形成新的复杂物种.
实现了西普洛素 (2.84微米) 和莱沃素 (0.21微米) 的低检测极限.
在人类血清中成功量化了这些药物.

结论:

一种由维生素B6衍生的新型AIEE发光剂成功合成.
开发的AIEEgen NPL显示出有前途的应用程序,用于敏感和选择性检测西普罗素和利沃素.
这种方法有可能在复杂的生物矩阵中进行药物分析,例如人体血清.

关键词:

一个AIEE的光源.这里是船只基地.这就是西普罗夫洛克萨辛 (Ciprofloxacin).光探测器的光探测器这种药物是levofloxacin.维生素B6的辅助因子

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