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热操纵和血清激素调节对大脑HSP70和HSP90基因表达在肉晚期胚胎发生的作用

Hamed KHasti¹, Ladan Emadi^1,2, Hahrzad Azizi³

¹Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.

Veterinary medicine and science

|April 2, 2025

概括

在暴露于热应激 (HS) 的 brojiler 胚胎中,色胺作为抗压剂起作用. 补充血清素增加了热冲击蛋白 (HSP70和HSP90) 的表达,减轻HS对胚胎大脑组织的影响.

科学领域:

动物科学动物科学
生物化学生物化学
生理学生理学生理学

背景情况:

由于生理上的限制,肉非常容易受到热应激 (HS).
血清素在神经发育和适应环境压力因素方面发挥着至关重要的作用.
热冲击蛋白 (HSP70和HSP90) 是细胞对抗压力的重要保护剂.

研究的目的:

研究HS对胚胎肉大脑中细胞应激反应的影响.
确定血清素在HS条件下调节压力反应中的作用.

主要方法:

受精的 brojler 蛋被用血清素或盐水处理,并接受受控的热应激.
在第18天收集了大脑组织,用于组织病理学和基因表达分析.
量化了HSP70和HSP90的mRNA表达.

主要成果:

热应激显著降低了胚胎大脑中的HSP70和HSP90基因表达.
血清激素治疗抵消了HS引起的HSP70和HSP90表达的下降.
血清激素通过在HS下升调HSP来表现出抗压力作用.

结论:

这项研究是第一个在HS下在 brojiler胚胎中显示减少大脑HSP70和HSP90表达.
血清激素通过在热应激期间增强HSP基因表达来起到抗压剂的作用.
需要进一步研究血清素对化后耐热性和性能的影响.

关键词:

高度服务提供者 (HSP)大脑大脑大脑的大脑大脑的胚胎的胚胎热应激是一种热应激.血清胺 (serotonin) 是一种神经元.

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