相关实验视频

Updated: Jul 16, 2026

Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells

Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells

Published on: June 19, 2013

一种用于计算机视觉分析真菌特征的设备,在辨别宿主抵抗位点方面,优于专家的定量手动显微镜

Surya Sapkota¹, Dani Martinez², Anna Underhill³

¹USDA-ARS Grape Genetics Research Unit, Geneva, New York, United States; sds322@cornell.edu.

|April 2, 2025

概括

一个新的高通量表型装置,黑鸟,迅速量化葡萄藤粉的耐药性. 这项技术加速了对REN13等抗性基因的发现,这对耐用葡萄品种至关重要.

科学领域:

植物病理学植物病理学
遗传学遗传学是一个
农业科学农业科学

背景情况:

准确的表型定型对于识别定量特征位点 (QTL) 至关重要,特别是那些具有轻微影响的特征位点.
葡萄树粉耐药性表型的先前方法是劳动密集型的,需要大量的显微镜时间.

研究的目的:

评估一个高通量表型装置 ("黑鸟") 用于葡萄藤粉的非破坏性量化.
通过使用先进的表型化数据,识别与耐药性相关的遗传位置.

主要方法:

利用"黑鸟"设备结合宏观摄影,机器人技术和卷积神经网络 (CNN) 来获取图像.
收集了时间序列的表型数据,从注射后2到9天 (dpi).
用RNase H2-amplicon测序 (rhAmpSeq) 标记器进行基因分析的综合表型化数据.

主要成果:

黑鸟实现了比手动显微镜高出60倍的吞吐量.
该装置使葡萄藤粉的非破坏性,时间序列表型化成为可能.
使用黑鸟生成的数据检测到了8号染色体上的REN13新型耐药性位点.
与手动显微镜 (15.8%在8dpi) 相比,CNN分析解释了更大的表型变异 (24.5%在4dpi的形,24.0%在9dpi的形).

结论:

黑鸟装置提供了一种快速有效的方法,用于葡萄藤粉状菌的表型.
REN13是"诺顿"品种中一种适度影响的耐药性位点,对繁殖有价值.
将黑鸟与遗传标记物集成,有助于QTL的发现,并可以增强耐药性基因堆叠,以对葡萄藤中持久的疾病管理.

关键词:

疾病耐药性疾病耐药性菌类病原体的真菌病原体遗传学遗传学是一个宿主寄生虫的相互作用技术技术技术技术

更多相关视频

Live-cell Imaging of Fungal Cells to Investigate Modes of Entry and Subcellular Localization of Antifungal Plant Defensins

Live-cell Imaging of Fungal Cells to Investigate Modes of Entry and Subcellular Localization of Antifungal Plant Defensins

Published on: December 24, 2017

Automated Measurement of Cryptococcal Species Polysaccharide Capsule and Cell Body

Automated Measurement of Cryptococcal Species Polysaccharide Capsule and Cell Body

Published on: January 11, 2018

相关概念视频

Rapid Identification of Pathogens

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...

Automated Microbial Diagnostics

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...