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在Xenopus laevis皮肤上的益生菌殖民导致皮肤微生物组和基因表达的短期变化

Joseph D Madison^1,2,3, Owen G Osborne⁴, Amy Ellison⁴

¹Center for Conservation Genomics, Smithsonian's National Zoo and Conservation Biology Institute, Washington, DC, USA.

Infection and immunity

|April 2, 2025

概括

对青施加的益生菌细菌改变了它们的皮肤微生物群落和免疫基因表达,最初的影响最为明显. 这些发现突出了两生物微生物组与免疫系统之间的复杂相互作用.

科学领域:

两动物免疫学两动物免疫学
微生物组研究的研究.
野生动物疾病生态学野生动物疾病生态学

背景情况:

两动物皮肤微生物群落影响疾病易感性.
益生菌疗法正在探索野生动物疾病管理,但它们对宿主微生物相互作用的影响尚不清楚.

研究的目的:

为了研究益生菌细菌如何影响皮肤微生物群落的组成和免疫基因表达在Xenopus laevis.
了解两生物微生物组和免疫系统的相互依赖.

主要方法:

应用了三种抗*Batrachochytrium*的细菌菌株 (两个是*Pseudomonas* spp.,一个是*Stenotrophomonas* sp.) 在*X. laevis*上.
通过16S rRNA基因测序量量化微生物群落.
使用定量逆转录酶聚合酶链反应 (RT-qPCR) 和皮肤转录组学评估免疫基因表达.

主要成果:

所有益生菌治疗都在X. laevis的皮肤上定居了3周,随着时间的推移,其丰度会变化.
接触益生菌改变了细菌群体的多样性,并减少了抗*Batrachochytrium*细菌的相对丰度.
免疫基因表达的变化,包括降低调控性T细胞标记物 (*FOXP3*) 表达,在暴露后的第1周最显著.

结论:

益生菌的应用显著改变两动物皮肤微生物群落和宿主免疫基因表达.
微生物群-免疫接口对于理解两动物疾病动态和生态进化过程至关重要.
这些发现强调两动物皮肤免疫力和微生物群落的相互依赖.

关键词:

两动物两动物疾病生态学生态学免疫反应的免疫反应.转基因组学是指转基因组学.微生物生态学微生物生态学微生物组是一个微生物组.益生菌是一种益生菌.转录组学转录组学是指转录组学.

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