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在法医FFPE分子分析中,是否充分考虑了预分析因素? 系统性审查揭示了对标准化程序的需求

Carlo Previderè¹, Serena Bonin², Calogero Cuttaia²

¹Department of Public Health, Experimental and Forensic Medicine, Section of Legal Medicine and Forensic Sciences, University of Pavia, 27100, Pavia, Italy.

International journal of legal medicine

|April 2, 2025

概括

法医分子尸检依赖于FFPE组织,但分析前的细节往往缺失. 这些参数的标准化报告对于法医病理学中可靠的DNA和RNA分析至关重要.

科学领域:

法医科学法医科学法医科学
分子生物学分子生物学
病理学病理学病理学

背景情况:

分子尸检通过将分子分析与传统发现相结合,增强了法医病理学.
在法医调查中,在回顾性分子DNA/RNA研究中,形式素固定的嵌 (FFPE) 组织至关重要.
分析前的变量 (死期时间,死后间隔,固定,储存) 显著影响核酸质量和下游分子测试可靠性.

研究的目的:

审查有关分子尸体解剖的最新法医文献中关于分析前参数的报告.
在已发表的研究中确定影响分子结果评估的关键缺失信息.
为法医病理学家提出标准化报告表,以提高数据的一致性.

主要方法:

在法医科学中对DNA和RNA分析进行50项选定研究的文献综述.
评估关键预分析参数的报告频率,包括死亡时间,尸检后的间隔和固定程序.
关于法医解剖组织样本的拟议标准化数据收集表格的开发.

主要成果:

只有34.9%的DNA研究和40.5%的RNA研究报告了基本的分析前参数.
关于痛苦的长度和甲素组织固定程序的重大遗漏被注意到.
这些报告漏洞阻碍了在法医案例工作中对分子测试结果的批判性评估和比较.

结论:

分析前参数的不一致报告损害了分子尸检发现的可靠性.
通过专门的报告表格进行标准化对于法医界来说至关重要.
实施标准化数据收集将提高法医病理学分子分析的可比性和有效性.

关键词:

在FFPE的FFPE.分子解剖的方法是分子解剖.核酸是核酸中的一种.在分析前进行预分析.标准化标准化标准化

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