西格莱克-15是猪流行性腹病毒感染的假定受体
Zhihua Feng1,2,3,4, Yajuan Fu3,4, Sheng Yang3,4
1Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, Fujian, 350117, China.
Cellular and molecular life sciences : CMLS
|April 2, 2025
概括
与酸结合的Ig样性莱克15 (Siglec-15) 作为猪流行性腹病毒 (PEDV) 的受体,增强病毒吸附. 向Siglec-15提供了针对PEDV感染的新治疗策略.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 猪流行性腹病毒 (PEDV) 在猪业中造成了相当大的经济损失.
- 进入宿主细胞的PEDV的确切机制仍然不完全理解.
- 酸作为共同受体的潜在作用表明酸结合蛋白的参与.
研究的目的:
- 调查酸结合性Ig类学蛋白15 (Siglec-15) 作为PEDV的潜在受体的功能.
- 为了阐明Siglec-15和PEDV组件之间的分子相互作用.
- 评估Siglec-15作为PEDV感染的治疗点.
主要方法:
- RNA测序 (RNA-Seq) 数据分析以确定潜在的宿主因素.
- 同免疫沉测试以确定蛋白质与蛋白质之间的相互作用.
- 免疫光显微镜可视化Siglec-15和PEDV的局部化.
- 使用Siglec-15淘汰赛小鼠来评估体内感染性.
- 开发并测试了一种针对Siglec-15的单克隆抗体,以确定其治疗疗效.
主要成果:
- 通过促进病毒吸附,Siglec-15被确定为增强PEDV感染的关键因素.
- 西格莱克-15直接与PEDV的S1亚单元和M蛋白结合.
- 在Siglec-15淘汰赛小鼠中,PEDV感染性显著降低.
- 一种向Siglec-15的单克隆抗体在体外和体内证明有效抑制PEDV感染.
结论:
- 西格莱克-15作为PEDV的受体或共受体,促进病毒的进入.
- 这种相互作用涉及Siglec-15与PEDV的S1亚单元和M蛋白结合.
- 西格莱克-15代表了一个有前途的新目标,用于开发针对PEDV的治疗干预措施.
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