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开发一种基于转子子子的基因组工程工具包,用于在Wolfiporia cocos中进行高效和可适应的基因修饰

Seungwoo Baek¹, Bogun Kim¹, Duleepa Pathiraja²

¹Department of Biotechnology, School of Life Science and Biotechnology, Korea University, Seoul 02847, Republic of Korea.

ACS synthetic biology

|April 2, 2025

概括

科学家们为Wolfiporia cocos开发了一套新的基因组工程工具包,这是一种被珍视的生物活性化合物的真菌. 这种工具提高了转化效率,并允许快速基因改造,推进了真菌生物技术.

科学领域:

菌生物技术是一种真菌生物技术.
合成生物学合成生物学
基因组工程是基因组工程.

背景情况:

真菌生物技术正在迅速发展,需要针对不同真菌菌株量身定制的基因组工程工具.
沃尔菲波利亚可可斯是一种以其生物活性化合物而闻名的有价值的真菌,但需要特定的遗传工具来操纵.

研究的目的:

开发和介绍一个基于转子子的基因组工程工具包,优化为Wolfiporia cocos.
提高转化效率,使W. cocos. 中的多重基因集成成为可能.
为了促进W. cocos.的快速和灵活的遗传原型化.

主要方法:

为W. cocos.开发一个基于转子子的工具包.
将多个基因组装成跨体体,以尾酒形式进行多重集成.
使用转录基因数据识别和验证本地W. cocos促进体.

主要成果:

该工具包显著提高了W. cocos. 的转化效率.
多复合基因整合和快速原型化是使用尾酒形式实现的.
通过基因组整合证实了跨世代稳定的基因表达.
确定了6个本地W. cocos促进体,其中2个在体中表现出强大的构成性表达.

结论:

开发的基于转子子子的工具包为W. cocos.中的合成生物学应用提供了多功能和高效的资源.
这种工具包使适应性基因修改成为可能,支持W. cocos.的生物技术潜力.
已识别的原生促进体可以用于精确控制工程W. cocos菌株的基因表达.

关键词:

在Tn5转位子上这种植物是Wolfiporia cocos.基底基因基因基因基因基因基因生物零部件生物零部件基因组工程是基因组工程.本地推广者本地推广者转化转化转化转化转化转化转化转化

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