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在Exserohilumturcicum效应器EtEC81重新编程玉米的替代拼接,并激活免疫力
Haiyue Yu1, Xuetao Shi2, Na Ning1
1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Cell reports
|April 2, 2025
概括
北方玉米叶病原体作用者可以重新编程植物mRNA拼接. Exserohilum turcicum effector 81 (EtEC81) 针对ZmEIP1改变玉米前mRNA剪接,影响植物免疫力.
科学领域:
- 植物病理学 植物病理学
- 分子生物学分子生物学
- 在RNA生物学,RNA生物学.
背景情况:
- 病原体效应者可以操纵宿主mRNA拼接,以逃避植物免疫力.
- 在调节宿主RNA拼接方面,Exserohilum turcicum效应者的作用以前是未知的.
- 北方玉米叶病 (NLB) 是一种影响玉米 (Zea mays) 生产的重大疾病.
研究的目的:
- 为了调查Exserohilum火效应剂是否干扰玉米RNA拼接.
- 阐明EtEC81影响宿主基因表达和免疫力的分子机制.
- 确定控制NLB的潜在目标.
主要方法:
- 对Exserohilum turcicum效应物的鉴定和描述 EtEC81.1.
- 分析EtEC81与玉米蛋白的相互作用,包括ZmEIP1.1.
- 转录组分析 (RNA测序) 以确定替代拼接事件.
- 在玉米植物中进行功能性测试,以评估EtEC81和ZmEIP1对拼接和防御的影响.
主要成果:
- 在Exserohilumturcicum效应器EtEC81调节玉米前mRNAs的替代拼接 (AS).
- EtEC81与玉米 EtEC81-交互蛋白1 (ZmEIP1) 进行物理相互作用,这是与玉米结合体相关的组成部分.
- 过度表达EtEC81或ZmEIP1导致119个常见事件的改变AS,这表明在诱导免疫反应中发挥了作用.
- EtEC81 负面调节 E. turcicum 病原性,而 ZmEIP1 积极调节防御反应.
结论:
- 在玉米中,Exserohilum turcicum效应器EtEC81针对ZmEIP1重新编程前mRNA剪接.
- 这种效应器-宿主相互作用为NLB病原体如何操纵宿主过程提供了机制基础.
- 了解这种相互作用为开发防止NLB的战略提供了潜在的目标.
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