环境盐度对Trachemys scripta elegans中MAPK-NFAT5通路的影响及其在度调节中的作用
Xin Niu1, Yingnan Lu1, Yue Yuan1
1Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences, Hainan Normal University, Haikou 571158, China.
概括
红耳滑 (Trachemys scripta elegans) 通过激活MAPK-NFAT5信号通路来适应盐水. 这种分子机制有助于调节透压,这对于在不断变化的沿海环境中生存至关重要.
科学领域:
- 生理学 生理学 生理学
- 分子生物学分子生物学
- 环境科学 环境科学
背景情况:
- 海平面上升 (SLR) 导致沿海淡水化,影响水生生物.
- 淡水海Trachemys scripta elegans对盐度有耐受性,但其度调节机制尚不清楚.
- 了解度调节对于预测物种适应环境变化的能力至关重要.
研究的目的:
- 为了研究Trachemys scripta elegans暴露于不同盐度水平的度调节的分子机制.
- 为了确定关键的信号通路和分子参与者参与的适应盐水环境.
主要方法:
- 对Trachemys scripta elegans暴露在5 PSU和15 PSU盐度中的RNA-seq数据的分析.
- 测量离子和尿素度,血和尿液中的透压.
- 评估水素 (AQP) 和载体蛋白水平,NFAT5和MAPK酸化通过西方抹杀和抑制剂测定.
主要成果:
- 暴露于盐度增加了离子和尿素的分泌,提高了透压.
- 在脏组织中观察到水素 (AQPs),离子载体和高压调节器NFAT5 (包括化NFAT5) 的升调.
- 证实了MAPK信号通路的激活,其抑制阻断了NFAT5和度调节基因转录.
结论:
- 甲状腺虫使用MAPK-NFAT5信号通路进行调节,以应对盐水条件.
- 这项研究阐明了乌中度调节适应的分子基础,为水生生物对环境化的反应提供了洞察力.
- 这些发现有助于了解沿海物种面临海平面上升的适应能力.
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