相关实验视频

Updated: May 16, 2025

Systemic Delivery of MicroRNA Using Recombinant Adeno-associated Virus Serotype 9 to Treat Neuromuscular Diseases in Rodents

Systemic Delivery of MicroRNA Using Recombinant Adeno-associated Virus Serotype 9 to Treat Neuromuscular Diseases in Rodents

Published on: August 10, 2018

系统性硬化症相关的骨肌损失患者的miRNAs的差异化调制

Federica Tambaro¹, Antonietta Gigante¹, Carmen Gallicchio¹

¹Department of Translational and Precision Medicine, Sapienza University of Rome, Rome, Italy.

European journal of internal medicine

|April 2, 2025

概括

系统性硬化症 (SSc) 与影响肌肉质量的改变microRNAs (miRNAs) 有关. 这些循环的miRNAs可能在SSc相关的骨肌肉损失中发挥作用,而不仅仅是营养不良.

科学领域:

生物化学生物化学
免疫学免疫学免疫学
遗传学遗传学是一个

背景情况:

系统性硬化症 (SSc) 是一种自身免疫性疾病,导致纤维化和血管炎症.
骨肌肉损伤影响高达22%的SSc患者,预测结果不佳.
在SSc中肌肉损伤的发病原因尚不清楚,可能涉及微RNA (miRNA) 调制.

研究的目的:

在SSc患者中识别差异调节的循环miRNA.
调查这些miRNA与身体组成变化的关联.
探索miRNAs与SSc.临床过程之间的关系.

主要方法:

使用RT-qPCR量化了循环中的miRNA水平.
用ELISA测量了转化生长因子β1 (TGF-β1) 蛋白质水平.
身体组成参数,包括无脂肪质量指数 (FFMI) 和相角 (PhA),通过生物电阻分析进行评估.

主要成果:

与对照组相比,在SSc患者中观察到miR-15b, -21, -29a, -29b和 -133a的下调;在SSc患者中观察到miR-206和 -486的上调.
miR-29b与TGF-β1负相关;miR-15b与PhA正相关.
特定的miRNAs (miR-206, -15b, -486, -133a) 与肌肉质量,身体组成,疾病阶段和自身抗体子集有关.

结论:

参与肌肉的miRNAs的差异调制发生在SSc患者身上.
这些发现表明,营养不良之外的机制有助于SSc.骨肌肉损失.
循环中的miRNAs可能代表SSc相关肌肉损伤的新生物标志物.

关键词:

肌肉性较低,肌肉性较低.微型网是什么意思肌肉消耗是指肌肉的消耗.萨尔科佩尼亚是什么意思萨尔科佩尼亚系统性硬化症系统性硬化症

更多相关视频

Imaging Features of Systemic Sclerosis-Associated Interstitial Lung Disease

Imaging Features of Systemic Sclerosis-Associated Interstitial Lung Disease

Published on: June 16, 2020

Quantitative Magnetic Resonance Imaging of Skeletal Muscle Disease

Quantitative Magnetic Resonance Imaging of Skeletal Muscle Disease

Published on: December 18, 2016

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...