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Renal Clearance

Renal Clearance

The glomerular filtration rate (GFR) is a critical marker of kidney function, reflecting the efficiency of filtration by the glomeruli. Renal clearance of specific substances, such as inulin or creatinine, is commonly used to measure GFR.
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相关实验视频

Updated: May 16, 2025

Low-Cost, Volume-Controlled Dipstick Urinalysis for Home-Testing

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Published on: May 8, 2021

一个被动流微反应器用于尿液肌素测试.

Dumitru Tomsa¹, Yang Liu¹, Amanda Stefanson¹

¹Department of Physics and Astronomy, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada.

Microsystems & nanoengineering

|April 2, 2025

概括

一个新的微流体芯片 (uCR-Chip) 能够快速地在护理地点测量慢性脏病 (CKD) 的尿蛋白质. 这种低成本,准确的测定与现有的白蛋白测试相结合,为实验室诊断提供了可行的替代方案.

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Last Updated: May 16, 2025

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科学领域:

生物标志物检测和微流体学
慢性病 (CKD) 的护理点诊断.

背景情况:

慢性病 (CKD) 构成了全球健康和经济上的重大负担.
目前的CKD诊断方法依赖于实验室测试生物标志物,如肌素.
现有的测试是受监管的,并且缺乏照顾点 (PoC) 可用于例行监测的可访问性.

研究的目的:

开发一种被动流微反应器,用于色度测量尿液肌素测量 (uCR-Chip).
通过与微流体尿蛋白试验进行整合,创建用于CKD评估的PoC试验.
为了实现快速,准确和可访问的CKD生物标志物量化.

主要方法:

开发了一种使用双相压力补偿 (2-PPC) 技术的被动流微反应器 (uCR-Chip).
采用微流体通道网络设计和优化观察窗口 (OW) 进行流体控制和信号稳定.
采用定制光罩生产和干膜光电阻光刻法,用于精确的芯片制造;通过USB显微镜平台进行检测.

主要成果:

在7分钟内实现了统一而稳定的检测信号.
证明了高达40毫米的动态线性检测范围,检测的下限 (LOD) 为0.521毫米.
用人工尿验证了测量,显示可接受的回收和低矩阵效应,与商业PoC系统相比.

结论:

开发的uCR-Chip为慢性病 (CKD) 评估提供了可行的PoC测试.
微反应器提供准确的肌素量化,满足临床精度要求.
这种技术平台有可能在护理点测量各种疾病生物标志物.