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克罗夫顿杂草 (Ageratina adenophora) 的染色体级基因组组合

Fuyan Liu^1,2, Lin Du³, Tingting Li¹

¹State Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy and Yangling Branch of China Wheat Improvement Center, Northwest A&F University, Yangling, 712100, Shaanxi, China.

Scientific data

|April 2, 2025

概括

研究人员已经对侵袭性克罗夫顿杂草 (Ageratina adenophora) 的三倍基因组进行了测序. 这些基因组数据为植物提供了至关重要的洞察力.

科学领域:

基因组学就是基因组学.
植物生物学植物生物学
生态生态学生态生态学

背景情况:

克罗夫顿杂草 (Ageratina adenophora) 是一种主要的入侵物种,造成生态破坏和经济损害.
有限的基因组数据阻碍了对其侵入性特征和进化动态的理解.

研究的目的:

为了执行Ageratina adenophora的三倍体基因组的新组装.
为未来对入侵物种的研究提供高质量的参考基因组.

主要方法:

利用长期阅读的PacBio Sequel测序来进行基因组组装.
集成的光学映射和Hi-C测序用于基因组架构和分辨率.
进行了BUSCO分析,以评估基因完整性和基因组注释.

主要成果:

成功组装了A. adenophora的三倍体基因组,其中包括51个染色体.
基因组大小大约为3.82 Gb,支架N50为70.8 Mb.
组装完成率达到97.71% (BUSCO),预测了123,134个蛋白质编码基因,并确定了76.44%的重复序列.

结论:

产生的高质量的参考基因组是研究A. adenophora.进化史的宝贵资源.
这些基因组数据将促进对驱动克罗夫顿杂草入侵性的适应机制的研究.

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The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.