在二次和持久性内牙炎感染中,根管和牙硫微生物群之间的关联
Dong Hyun Park1, Euon Jung Tak2, Ok-Jin Park1
1Department of Oral Microbiology and Immunology, DRI, School of Dentistry, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, Republic of Korea.
Scientific reports
|April 2, 2025
概括
二次性/持久性内牙感染 (SPEIs) 难以诊断. 这项研究确定了根管和牙口袋中的关键细菌,表明了潜在的生物标志物,以更轻松地检测和监测SPEI.
科学领域:
- 微生物学 微生物学
- 牙周内科医院 牙周内科医院 牙周内科医院
- 分子诊断学 分子诊断学
背景情况:
- 二次性/持久性内牙感染 (SPEIs) 是由于根管治疗失败而引起的,导致持续的顶端牙周炎.
- 目前对SPEIs的诊断方法缺乏敏感性和特异性,因此需要改进检测和监测工具.
研究的目的:
- 为了与SPEI比较和描述牙根通道和牙突中的微生物群.
- 使用下一代测序 (NGS) 识别关键病原体作为SPEIs的潜在诊断生物标志物.
主要方法:
- 对25名接受非手术再处理的患者30颗牙的90个样本进行分析.
- 细菌DNA提取,16S rRNA基因V3-V4区域放大,以及Illumina MiSeq测序.
- 使用安普利康序列变异 (ASV) 识别细菌组成.
主要成果:
- 微生物群的组成在根通道 (Acinetobacter,Veillonella) 和牙 (Streptococcus,Actinomyces) 之间有显著差异.
- 在两个地点都发现了特定物种 (例如,Shuttleworthella satelles,Klebsiella pneumoniae),表明潜在的微生物迁移.
- NGS确定了16个类,182个属和390个物种,提供了一个全面的微生物概况.
结论:
- 在受SPEI影响的牙的根通道和牙突中存在明显的微生物特征.
- 在硫液中检测到的某些关键病原体可以作为SPEI诊断和监测的可访问生物标志物.
- 研究结果支持针对SPEI开发有针对性的诊断和治疗策略.
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