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单细胞转录组分析揭示了MIF对多发性骨髓瘤中髓衍生的抑制细胞的影响

XiaoDong Zhao¹, JingXin Zhou¹, JinRong Yao¹

¹Department of Hematology, The Affiliated Suqian First People's Hospital of Nanjing Medical University, SuQian, Jiangsu, China.

International journal of laboratory hematology

|April 3, 2025

概括

巨细胞迁移抑制因子 (MIF) 在多发性骨髓瘤 (MM) 患者中升高,与骨髓中的骨髓衍生抑制细胞 (MDSC) 相对应. 本研究确定了不同的MIF表达MDSC子集及其在MM中的通信途径.

科学领域:

免疫学免疫学免疫学
血液学血液学血液学
癌症生物学癌症生物学

背景情况:

多发性髓瘤 (MM) 是一种致命的血液性恶性瘤.
骨髓衍生抑制细胞 (MDSCs) 显著影响MM骨髓微环境.
巨细胞迁移抑制因子 (MIF) 是一种调节免疫反应的炎症性细胞因子.

研究的目的:

调查MM骨髓微环境中的MIF和MDSC之间的关系.
在MM患者中描述表达MIF的MDSC子集.

主要方法:

单细胞RNA测序 (scRNA-seq) 的MM和正常样本 (GSE124310数据集).
使用CellMarker 2.0.0进行细胞类型注释.
使用CellChat推断细胞细胞通信通路.
流细胞计用于表征细胞标记物.

主要成果:

scRNA-seq确定了10个细胞子集,MDSC根据MIF表达被分为MIF+和MIF-子集.
在MM和正常组之间观察到MIF+和MIF-MDSC的不同比例.
MIF+ MDSCs显示了低的RETN-CAP1通路与血细胞的信号传递,而MIF-MDSCs显示了高的信号传递.
流细胞计证实了MM MDSCs中RETN,Arg-1和iNOS的增加,在MIF+MDSC中表达更高. 在MM血细胞中,CAP1升高.

结论:

在MM患者中,MIF是上调调的.
在MM骨髓微环境中,MIF表达与MDSC相关.
不同的MDSC子集及其通信途径,如RETN-CAP1,与MM进展有关.

关键词:

骨髓微环境骨髓微环境巨细胞迁移抑制因子的抑制因子多发性骨髓瘤是多发性骨髓瘤的一种.骨髓蛋白衍生的抑制细胞.一个单细胞RNA测序.

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