相关实验视频

Updated: Jun 17, 2026

Virus-induced Gene Silencing VIGS in Nicotiana benthamiana and Tomato

Virus-induced Gene Silencing VIGS in Nicotiana benthamiana and Tomato

Published on: June 10, 2009

番茄黄叶卷曲病毒诱导的番茄和烟草植物基因表达模式的比较

Chenwei Zhang¹, Xin Jia¹, Xing Han¹

¹Modern Agricultural Science and Technology Laboratory, Department of Agronomy and Food Science, Shijiazhuang University, Shijiazhuang 050035, China.

The plant pathology journal

|April 3, 2025

概括

番茄黄色叶子卷曲病毒 (TYLCV) 感染导致番茄和烟草植物的严重症状. 沉默特定的转录因子降低了病毒水平,提供了对植物耐药性机制的见解.

科学领域:

植物病理学植物病理学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

番茄黄色叶子卷曲病毒 (TYLCV) 造成了严重的农业损失.
TYLCV感染了重要的作物物种,如番茄 (Solanum lycopersicum L.) 和烟草 (Nicotiana benthamiana).

研究的目的:

研究番茄和烟草植物对TYLCV感染的分子和生理反应.
为了确定关键的基因和途径参与植物防御TYLCV.

主要方法:

利用TYLCV的构造感染性克隆来控制植物感染.
在受感染的番茄和烟草植物中分析了基因表达特征 (转录组学).
通过基因沉默实验,研究了RAV转录因子在抗病毒防御中的作用.

主要成果:

TYLCV感染引起了包括化,叶子卷曲,矮化,反应性氧物种积累和亡在内的特征性症状.
在两种物种中都观察到数千个基因的显著上调,包括转录因子 (MYB,MADS-box,RAV),激酶,热冲击蛋白和细胞染色体P450s.
沉默番茄中的RAV转录因子导致TYLCV丰富性的减少,突出其在抗病毒信号传递中的作用.

结论:

感染TYLCV会触发易受植物复杂的防御反应,涉及广泛的基因表达变化.
RAV转录因子在酸介导的抗病毒信号传递和植物对TYLCV的耐药性中起着至关重要的作用.
了解这些分子机制可以为开发抗TYLCV作物品种的策略提供信息.

关键词:

尼科蒂亚纳 (Nicotiana benthamiana) 是一种植物.这种植物是Solanum lycopersicum L. 的.植物防御机制的防御机制番茄黄色叶子卷曲病毒 (TYLCV)

更多相关视频

Characterization of Complex Systems Using the Design of Experiments Approach: Transient Protein Expression in Tobacco as a Case Study

Characterization of Complex Systems Using the Design of Experiments Approach: Transient Protein Expression in Tobacco as a Case Study

Published on: January 31, 2014

Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun

Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun

Published on: April 18, 2014

相关概念视频

Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...