在Capra hircus中内源性逆转录病毒衍生的转录的多组织转录组特征
Ming-Di Li1,2, Hu-Rong Li1, Shao-Hui Ye1
1Department of Animal Breeding and Reproduction, College of Animal Science and Technology, Yunnan Agricultural University, Kunming, China.
Frontiers in genetics
|April 3, 2025
概括
可转移元素 (TE) 占山羊基因组的近一半. 这项研究揭示了内源逆转录病毒 (ERV) 在山羊胚胎中高度活跃,对病毒感染作出反应,影响附近的基因表达.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 生物信息学是一种生物信息学.
背景情况:
- 可转移元素 (TE) 是重复的DNA序列,构成了哺乳动物基因组的很大一部分.
- 虽然经常被沉默,但在特定的生理条件下,某些TE,特别是内源逆转录病毒 (ERV),可以表达.
- 像山羊 (Capra hircus) 这样的研究较少的物种中TEs的表达模式以前尚不清楚.
研究的目的:
- 综合分析山羊中TE和ERV的基因组和转录组特征.
- 在各种山羊组织和条件中研究TE和ERV的表达模式.
- 探索ERV与邻近基因的共同表达,以了解调节机制.
主要方法:
- 在山羊基因组类,家族和子家族中TE分布的系统注释.
- 在亚家族和位置层面的多种组织中分析TE衍生转录表达.
- 在生理 (胚胎发育) 和病理 (病毒感染) 条件下,ERV衍生的差异表达分析读取.
- 探索ERV读取和近位基因之间的共同表达.
主要成果:
- 确定了大约800个TE子家族,占山羊基因组的49.1%.
- 来自TE的读数占转录组的10%,在整个组织中相对一致.
- 在不同组织中,ERV的表达有所不同,在16细胞胚胎中表现出高活性,并在肺部,脏,皮和免疫细胞中对RNA病毒感染的反应中表现出高活性.
- 特定的ERVK元素 (CapAeg_1.233) 在子宫内膜发育和感染期间显示出失调,与OAS1和TMPRSS2.2.等近位基因共同表达.
结论:
- 为山羊组织生成了全面的ERV转录组.
- 确定了在山羊的不同生理和病理状态中激活的特定ERV.
- 证明了近邻基因表达对附近ERV活动的影响.
更多相关视频
13:07Determining 3'-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells
Published on: January 30, 2019
9.2K
12:53Bidirectional Retroviral Integration Site PCR Methodology and Quantitative Data Analysis Workflow
Published on: June 14, 2017
10.7K
相关概念视频
Retroviruses
12.1K
Retroviruses and retrotransposons both insert copies of their genetic elements into the genome of the host cell. Thus, the viral genes are passed on when the host genome is replicated or translated. A typical retroviral DNA sequence contains 3-4 genes that encode the different proteins required for its structural assembly and function as a molecular parasite. This DNA is transcribed into a single mRNA, which is very similar in structure to conventional mRNAs, i.e., it is capped at the 5’...
12.1K
Non-LTR Retrotransposons
11.3K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
11.3K
LTR Retrotransposons
17.3K
LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less abundant in mammals compared to other class I transposable elements. About 8 percent of human genomic DNA comprises LTR retrotransposons. Some of the common examples of LTR retrotransposons are Ty elements in yeast and Copia elements in Drosophila.
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
17.3K
Retrovirus Life Cycles
45.4K
Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...
45.4K
Mechanisms of Retrovirus-induced Cancers
5.0K
Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
5.0K
