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检测范素敏感的Enterococcus faecium分离物中的vanA基因:对额外测试的含义

Victoria Jordan^1,2, Hemalatha Varadhan^1,2

¹Department of Microbiology, NSW Health Pathology, John Hunter Hospital, New Lambton Heights, NSW 2305, Australia.

Access microbiology

|April 3, 2025

概括

在6%的Enterococcus faecium分离物中检测到沉默的万科米辛耐药性. 这一发现凸显了在缺乏快速基因型检测方法的实验室中识别耐药性的挑战.

科学领域:

微生物学微生物学
分子生物学分子生物学
临床诊断临床诊断临床诊断

背景情况:

肠球菌 (Enterococcus faecium) 是医院感染的常见原因之一.
Enterococcus faecium中的万科米辛耐药性是一个重大的公共卫生问题.
沉默的万科米辛耐药基因可以导致未被发现的耐药性.

研究的目的:

在表型敏感的Enterococcus faecium分离物中确定沉默的万科米抗性基因 (vanA和vanB) 的患病率.
评估基因型检测对识别无声抗性的有用性.
突出临床实验室在检测这种耐药性的过程中所面临的挑战.

主要方法:

对Enterococcus faecium分离物的表型敏感性测试.
基因型测试使用Cepheid的vanA和vanB基因的vanA/vanB PCR测定.
分析隔离数据以确定静音电阻.

主要成果:

6%的表型敏感的Enterococcus faecium分离物中含有沉默的vanA基因.
该研究确定了在表型上没有表现出万科米辛耐药性的分离物中存在vanA基因的存在.
在摘要中没有报告vanB基因.

结论:

沉默的万科米辛耐药性基因存在于菌菌 (Enterococcus faecium) 分离物的子集中.
这些沉默基因的临床相关性需要进一步调查.
缺乏快速,随机访问的基因型方法,对准确及及时的实验室检测万科米辛耐药性提出了挑战.

关键词:

瓦纳 (vanA) 的意思是这就是VanBB的意义.抗万科胺素的肠球菌 (Enterococci) 是一种耐药性菌.菌素变异性肠球菌.

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