在自发发酵的脉冲蛋白分离物中识别微生物蛋白质和蛋白质组的变化
Prem Prakash Das1, Caishuang Xu1, Yuping Lu1
1National Research Council Canada, 110 Gymnasium Pl, Saskatoon, SK S7N 0W9, Canada.
Food chemistry. Molecular sciences
|April 3, 2025
概括
植物性蛋白质的自发发酵,如小豆,法巴豆和豆,引入微生物蛋白质. 这一过程水解了本地脉冲蛋白,可能会影响成分的功能.
科学领域:
- 食品科学 食品科学 食品科学
- 蛋白质组学是指蛋白质组学.
- 微生物学 微生物学
背景情况:
- 豆类提供可持续的植物性蛋白质,但往往缺乏理想的感官和功能性质.
- 发酵是一种增强食品加工的自然方法,但自发发酵对脉冲蛋白成分的影响尚不清楚.
研究的目的:
- 为了研究小,法巴豆和豆蛋白质分离物的自发发酵过程中蛋白质水平的变化.
- 识别微生物蛋白质并评估它们对整体蛋白质特征的贡献.
主要方法:
- 毛细管凝电泳 (CGE) 用于蛋白质大小分布分析.
- 液体染色学与双重质谱学 (LC-MS/MS) 结合,用于蛋白质组分析.
- 对经过自发发酵的小豆,法巴豆和豆蛋白质分离物的分析.
主要成果:
- 从各种细菌和真菌物种的蛋白质被确定在所有的脉冲基板.
- 微生物蛋白质的丰富性和多样性在自发发酵过程中显著增加.
- 观察到本地脉冲蛋白的水解,与微生物蛋白质含量增加相关.
结论:
- 自发发酵将显著的微生物蛋白质含量引入脉冲分离物中.
- 观察到的蛋白质水解表明,由于微生物活动,脉冲蛋白的功能发生了改变.
- 需要进一步的研究才能充分理解对脉冲性成分应用的影响.
关键词:
豆是什么意思 豆是什么意思法巴豆 (Faba bean) 是一种豆类.食品经济学 (Foodomics) 是一个关于食品的学科.绿色的镜片绿色的镜片蛋白质组是蛋白质组的组成部分.固态发酵固态发酵是什么更多相关视频
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