叶子切割和暴露时间改变了不可逆转光合作用热值的估计
Margaux Didion-Gency1,2,3, Alice Gauthey4, Kate M Johnson1,2,3
1Ecological and Forestry Applications Research Center CREAF, Bellaterra, Spain.
Plant, cell & environment
|April 3, 2025
概括
植物的耐热性取决于温度值,但方法各不相同. 长时间的热暴露和ex-vivo方法降低了临界温度,这表明光系统II的45°C以上的不可逆转损伤.
科学领域:
- 植物生理学 植物生理学
- 光合作用研究研究光合作用.
- 适应气候变化 适应气候变化
背景情况:
- 评估植物的耐热性对于了解气候变化影响至关重要.
- 在植物中确定热值的常用方法缺乏标准化和比较.
- 在临界温度下光合作用损伤的可逆性仍然不太清楚.
研究的目的:
- 为了比较不同的方法来评估工厂的热门值.
- 为了研究热暴露时间对热耐受性的影响.
- 为了确定在地中海针叶树的关键温度下,光合作用损伤是否不可逆转.
主要方法:
- 确定光系统II的临界温度 (Tcrit),50%的降温温度 (T50) 和最大可容忍温度 (Tmax).
- 使用了in-vivo,ex-vivo和ex-vivo连续热暴露方法 (持续时间为15分钟和30分钟).
- 测量暗适应光 (Fv/Fm) 和气体交换后热应激4天,以评估恢复.
主要成果:
- 与较短的暴露时间 (15分钟) 相比,更长的暴露时间 (30分钟) 始终导致更低的Fv/Fm,T50和Tmax.
- 外体检测方法的T50和Tmax值低于体内检测.
- 跨物种,暴露时间和方法的Tcrit保持一致,这表明一个可逆值.
结论:
- 暴露时间和测量方法显著影响工厂的热值的确定.
- Tcrit似乎是光系II的可逆热值.
- 在研究的松树物种中,光合作用机器的不可逆转损伤发生在T50 (约45°C) 以后.
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