对小分子的离子移动性数据采集,校准和处理的评估:对漂流管和移动波方法的跨平台评估
James N Dodds1, Lucie C Ford2, Jack P Ryan1
1Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27514, United States.
Journal of the American Society for Mass Spectrometry
|April 3, 2025
概括
离子移动光谱仪 (IMS) 仪器需要协调才能进行可靠的环境和生物分析. 与漂流管IMS-MS相比,MOBILion MOBIE在异构体分离方面提供了更高的分辨率,有助于曝光学和生物标志物发现.
科学领域:
- 分析化学 分析化学
- 频谱测量是一种光谱测量.
- 生物分子分析
背景情况:
- 离子移动光谱 (IMS) 越来越多地用于环境和生物分析.
- 协调和理解不同的IMS工具对于广泛采用至关重要.
- 新的IMS平台专注于提高分辨能力,以分离类似的分子.
研究的目的:
- 为了评估MOBILion MOBIE仪器的性能.
- 为了将MOBIE与商用漂流管IMS-MS (Agilent 6560) 进行比较.
- 评估IMS-MS数据的数据提取和分析管道.
主要方法:
- 对MOBIE和Agilent 6560.的比较性能的评估.
- 尽可能匹配运行条件.
- 使用LC-IMS-MS.使用扫描速度,分辨率 (Rp),检测极限 (LOD) 和异构体分离的评估.
- 在基于细胞的模型中对 perfluoroalkyl 物质 (PFAS) 的分析.
主要成果:
- 这两种仪器在IMS-MS图像生成方面表现出相似的扫描速度.
- MOBIE的碰撞横截面 (CCS) 值与漂流管值有≤1%的差异.
- 在以细胞为基础的样本中实现了对PFAS定量化的可比检测极限.
- 莫比表现出明显更高的分辨率 (200-300相比45-60CCS/ΔCCS) 比阿吉伦特6560.
- 在MOBIE上更高的Rp可以检测更多的PFAS异构体.
结论:
- 对于需要高分辨率的高级分析工作流程,MOBILion MOBIE显示出有前途的承诺.
- 它对同位素分离的能力有利于化学曝光学和生物标志物发现.
- 进一步发展数据分析方法对于先进的IMS平台的日常采用至关重要.
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