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巨细胞蛋白二硫化异硫酶增加了莱什曼亚马逊菌的感染

Guilherme M P Carrara¹, Guilherme A Souza-Silva¹, Tania C B D Reis²

¹Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, São Paulo, Brazil.

Cell biology international

|April 3, 2025

概括

表面蛋白二硫化异构酶 (PDI) 增强了Leishmania amazonensis寄生虫在体外被巨细胞吸收. 然而,小鼠的PDI过度表达并没有显著改变感染严重程度,这表明复杂的免疫相互作用.

科学领域:

免疫学免疫学免疫学
寄生虫学的寄生虫学
细胞生物学细胞生物学

背景情况:

莱什曼尼亚种类 (Leishmania spp.) 是一种在巴西,L. amazonensis 是一种普遍存在的皮肤热带物种.
巨细胞化对于莱什曼菌感染至关重要,涉及各种细胞表面蛋白质.
以前的研究将巨细胞表面蛋白二硫化异构酶 (PDI) 与莱什曼尼亚婴儿菌的细胞增多联系起来.

研究的目的:

研究表面蛋白二硫化异构酶 (PDI) 在巨细胞消化症和莱什马尼亚 (L.) amazonensis.感染中的作用.
评估PDI过度表达在转基因小鼠对L. amazonensis感染动态的影响.

主要方法:

使用过度表达PDI (TgPDIA1) 的转基因FVB/NJ小鼠.
通过流细胞计验证了巨细胞上PDI的存在.
评估PDI调节后的巨细胞感染和zymosan颗粒吸收 (抗PDI抗体,PDI过度表达).
在野生型和TgPDIA1小鼠中使用露西法酶表达寄生虫进行了感染的体内成像.

主要成果:

抗体介导的表面PDI阻塞减少了巨细胞中的L. amazonensis感染.
巨细胞中PDI的过度表达增加了寄生虫的粘附和随后的感染.
过度表达PDI的巨细胞表现出对齐莫桑颗粒的增强吸收.
在体内,PDI过度表达并没有导致脚病变或寄生虫负担的显著差异.

结论:

表面PDI在体外促进L. amazonensis的细胞和巨细胞的感染.
在体内PDI过度表达对Leishmania感染的影响是复杂的,并可能被其在免疫系统中的广泛作用所掩盖.

关键词:

莱什曼亚亚马逊人种 (Leishmania amazonensis) 是一个亚马逊人种.在PDIA1中,感染的感染感染.发酵细胞的形成发酵细胞的形成带体莱什曼病 - - 带体莱什曼病

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