Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

1.8K
1.8K
Protein Folding01:22

Protein Folding

117.0K
Overview
117.0K
Protein-protein Interfaces02:04

Protein-protein Interfaces

12.4K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
12.4K
Protein Complex Assembly02:41

Protein Complex Assembly

10.5K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
10.5K
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

5.7K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
5.7K
Protein Organization01:24

Protein Organization

6.2K
Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence....
6.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Sustainable Biopolymer Based on Tung Oil and Tung Free Fatty Acids for Hair Tensile Enhancement.

ACS applied bio materials·2026
Same author

Discovery amidst Artificial Intelligence: Protein-Receptor Interactions.

Biochemistry·2026
Same author

Modular protein frameworks via supramolecular synthons.

Biophysical reviews·2025
Same author

Next Generation Hosts for Protein Recognition, Assembly and More.

Chemistry (Weinheim an der Bergstrasse, Germany)·2025
Same author

High Shear Formation of Covalently Assembled 2D Sheets of Fullerene C<sub>60</sub> under Continuous Flow.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

High Shear Conversion of Elemental Bismuth in Water Under Air to 2D Bismuth Oxycarbonate.

Chemistry (Weinheim an der Bergstrasse, Germany)·2025
Same journal

Total Synthesis and Structural Revision of Tetracyclic Diterpenoid (±)-Papililone A and (-)-Papililone A.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Light-Powered Atroposelective Ratcheting via Excited-State Donor-Acceptor Interactions.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Modular One-Pot Access to π-Expanded Tetrakis(Phenothiazinyl)-Silanes With Broadly Tunable Redox and Emission Properties.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same journal

pH-Tolerant Tripeptide Coacervates as Biomimetic Catalytic Microreactors.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Nano-Nickel Pinned Defective MoS<sub>2</sub> Heterostructures via Ball Milling for Improved Hydrogen Evolution.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Hollow NiCo-LDH Nanocage Derived From ZIF-67 as an Efficient Catalyst for the Thermal Decomposition of Ammonium Perchlorate.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
查看所有相关文章

相关实验视频

Updated: May 23, 2025

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
09:34

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

7.1K

为模块化蛋白质框架制造和破坏超分子合成子

Niamh M Mockler1, Colin L Raston2, Peter B Crowley1

  • 1School of Biological and Chemical Sciences, University of Galway, University Road, Galway, H91 TK33, Ireland.

Chemistry (Weinheim an der Bergstrasse, Germany)
|April 3, 2025
PubMed
概括
此摘要是机器生成的。

阳离子卡力沙林介导蛋白质组装. 在Ralstonia solanacearum的乳素组合中,用酸[8]arene替换酸[6]arene控制了晶体形成,证明了synthon的耐用性.

关键词:
亲和度标签 亲和度标签 亲和度标签晶体工程是什么 晶体工程一个宏观周期的宏观周期.认可是一种认可.自动组装的自动组装机

更多相关视频

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
10:01

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro

Published on: April 8, 2020

5.8K
Synthesis of a Water-soluble Metal&#8211;Organic Complex Array
06:40

Synthesis of a Water-soluble Metal–Organic Complex Array

Published on: October 8, 2016

9.8K

相关实验视频

Last Updated: May 23, 2025

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
09:34

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

7.1K
Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
10:01

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro

Published on: April 8, 2020

5.8K
Synthesis of a Water-soluble Metal&#8211;Organic Complex Array
06:40

Synthesis of a Water-soluble Metal–Organic Complex Array

Published on: October 8, 2016

9.8K

科学领域:

  • 超分子化学 超分子化学
  • 蛋白质结晶学 蛋白质结晶学
  • 材料科学是一种材料科学.

背景情况:

  • 阳离子素是指导蛋白质自我组装成晶体结构的关键.
  • 拉尔斯托尼亚松 (Ralstonia solanacearum lectin,RSL) 呈现了与p-sulfonato-calix[8]arene (sclx8) 的pH依赖性共结晶,形成了多种多态.
  • sclx8二分子作为一个超分子合成子,影响得到的晶体结构.

研究的目的:

  • 为了研究用p-phosphonato-calix[6]arene (pclx6) 替换sclx8对RSL组件的影响.
  • 为了确定pclx6是否可以产生单一的,稳定的共晶形式.
  • 在蛋白质晶体工程中探索calixarene类型和synthon耐久性的作用.

主要方法:

  • 在各种pH值和结晶条件下使用RSL和pclx6的共同结晶实验.
  • 由此产生的RSL-pclx6共晶体的结构分析.
  • 使用RSL-sclx8共晶的比较研究.
  • 使用突变RSL与宏循环结合的亲和度标签的研究.
  • 用RSL,pclx6,和sclx8进行三级混合实验.

主要成果:

  • RSL-pclx6共结晶始终产生单晶形式,不论pH值或条件如何,与sclx8形成鲜明对比.
  • pclx6二极管证明更耐用,决定了组装和多态选择.
  • 一个带有亲和标签的突变RSL形成了一个改变的,多孔的框架与pclx.
  • 三级混合物在RSL,pclx6和sclx8之间显示了pH驱动的自我排序.

结论:

  • 素的类型 (酸与硫酸) 显著影响了超分子合成耐久性和蛋白质组装结果.
  • 卡利沙合成选择为控制蛋白质晶体工程和多态选择提供了一个强大的策略.
  • 使用蛋白质和超分子合成子的"混合匹配"方法是设计基于蛋白质的新材料的有希望的途径.