相关实验视频
Updated: May 16, 2025

07:33
Intralymphatic Immunotherapy and Vaccination in Mice
Published on: February 2, 2014
23.0K
复合性低过敏性猫类过敏疫苗
Daria Trifonova1,2, Mirela Curin1, Margarete Focke-Tejkl1
1Department of Pathophysiology and Allergy Research, Division of Immunopathology, Center for Pathophysiology, Infectiology, and Immunology, Medical University of Vienna, Vienna, Austria.
Allergy
|April 3, 2025
概括
新的分子疫苗 (PreS-Cat 1-5) 结合了关键的猫过敏原,为猫过敏免疫疗法带来了希望. 这些低过敏性融合蛋白诱导了强大的阻断抗体,提供了潜在的更有效的AIT选择.
科学领域:
- 免疫学 免疫学 免疫学
- 过敏原特定免疫疗法 (AIT)
- 开发分子疫苗的开发工作.
背景情况:
- 猫过敏是一种常见的疾病,Fel d1,Fel d4和Fel d7被确定为主要的过敏原.
- 目前的过敏原特异性免疫疗法 (AIT) 方法需要改进,需要开发新的分子形式.
研究的目的:
- 开发和评估用于猫过敏的新型低过敏性复合融合蛋白 (PreS-Cat 1-5) AIT.
- 评估这些PreS-Cat蛋白质在诱导IgE阻断抗体和调节免疫反应方面的免疫性和有效性.
主要方法:
- 用患者IgE结合抑制试验对Feld4和Feld7过敏原进行了皮层映射.
- 设计了五种重组融合蛋白 (PreS-Cat1-5),将主要猫类过敏原 (Fel d1,Fel d4,Fel d7) 的低过敏性合到一种 PreS 载体的乙型肝炎病毒 (HBV) 中.
- 蛋白质被表达在大肠杆菌中,被净化,并进行了表征. 通过ImmunoCAP和基细胞激活试验证实了低过敏性. 在子中评估了免疫性,将抗体诱导和IgE阻断能力与授权的AIT疫苗进行比较. 在周围血液单核细胞 (PBMC) 培养物中评估了T细胞反应和细胞因子诱导 (IL-10).
主要成果:
- 再组合的低过敏性PreS-Cat 1-5蛋白质已成功生产和表征.
- 与常规AIT疫苗的多剂量相比,在子中用PreS-Cat 1-5进行的两次免疫接种诱导了针对Feld4和Feld7的优异IgE阻断抗体.
- 针对PreS-Cat的特异性抗体有效抑制了基细胞的激活. 与本地猫过敏原相比,PreS-Cat 5和PreS-Cat 1在过敏患者的PBMC中诱导了显著更高的IL-10,一种抗炎性细胞因子.
结论:
- 普雷斯卡特5和普雷斯卡特1是对猫过敏AIT的非常有前途的分子疫苗候选人.
- 这些单链融合蛋白有效地结合了关键的猫类过敏原 (Fel d1,Fel d4,Fel d7) 并显示出改善免疫治疗结果的巨大潜力.
相关概念视频
Allergic Reactions
27.2K
Overview
27.2K
Antibody Structure
58.6K
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
58.6K
Cross-reactivity
30.9K
Overview
30.9K
Allergic Drug Reactions
799
Allergic reactions related to drugs are hypersensitivity responses driven by the immune system and bear no connection to the drug's therapeutic action. While drugs in isolation do not trigger an immune response, they can interact with endogenous proteins to form antigens. These antigens stimulate lymphocytes to produce antibodies. IgE-type antibodies attach themselves to mast cells. Upon subsequent exposure to the same stimulus, the antigen-antibody interaction is initiated, unleashing...
799
Hybridoma Technology
13.8K
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
13.8K
Hypersensitivities
426
Hypersensitivity, also known as a hypersensitivity reaction or allergic reaction, is a condition where the body's immune system reacts abnormally to a foreign substance. Such substances, that cause hypersensitivity are referred to as an allergen, could be something typically harmless to most people, like pollen or certain foods.
Types of Hypersensitivities
Hypersensitivity reactions are categorized into four types: Type 1, Type 2, Type 3, and Type 4. Each type has a distinct mechanism...
Types of Hypersensitivities
Hypersensitivity reactions are categorized into four types: Type 1, Type 2, Type 3, and Type 4. Each type has a distinct mechanism...
426

