2-乙基二酸盐影响人类上腺 (H295R) 细胞中的类固醇生成和脂质组特征
Chander K Negi1, Darshak Gadara1, Lola Bajard1
1RECETOX, Faculty of Science, Masaryk University, Kotlarska 2, 61137 Brno, Czech Republic.
Chemical research in toxicology
|April 3, 2025
概括
2-乙烯二酸 (EHDPP) 扰乱了人类上腺细胞中的激素产生和脂质平衡. 这种有机酸盐阻燃剂 (OPFR) 显示出对生殖健康的潜在内分泌干扰作用.
科学领域:
- 环境毒理学环境毒理学
- 内分泌学 在内分泌学.
- 细胞生物学 细胞生物学
背景情况:
- 人们越来越担心化学物质暴露和生殖健康问题.
- 有机酸盐阻燃剂 (OPFR) 被确定为潜在的生殖毒素.
- 2-乙烯二酸 (EHDPP) 是一种广泛使用的OPFR,需要进行毒理学评估.
研究的目的:
- 调查EHDPP的内分泌干扰潜力.
- 描述EHDPP对人类上腺皮细胞中的类固醇生成的影响.
- 分析EHDPP对脂质资料和基因表达的影响.
主要方法:
- 暴露人类H295R上腺皮细胞于EHDPP (1和5μM) 48小时.
- 测量激素的产生 (孕激素,安德罗二,,雌激素,皮质醇,阿尔多斯特).
- LC-MS/MS脂质组学,基因表达分析 (皮质类固醇生成,脂质代谢,线粒体动力学).
主要成果:
- 暴露于EHDPP会增加雌激醇,皮质醇和阿尔多斯特分泌.
- 观察到关键类固醇基因 (CYP11B2,CYP21A1,3β-HSD2,17β-HSD1) 的上调.
- EHDPP破坏了脂质平衡,改变了胆固醇,脂,脂和甘油三;影响了线粒体动力学.
结论:
- 在体外,EHDPP会破坏上腺类固醇生成和脂质平衡.
- EHDPP表现出潜在的内分泌干扰作用,影响生殖健康.
- 对EHDPP的环境和健康风险进行进一步调查是有必要的.
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