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主体ZAP活动与灵长类隐形病毒中CpG抑制水平相关

Rayhane Nchioua¹, Dorota Kmiec¹, Veronika Krchlikova²

¹Institute of Molecular Virology, Ulm University Medical Center, Ulm 89081, Germany.

Proceedings of the National Academy of Sciences of the United States of America

|April 3, 2025

概括

指抗病毒蛋白 (ZAP) 通常可以抑制病毒CpG二核酸. 然而,特定的子ZAP变体表现出减少的活性,导致类免疫缺陷病毒 (SIV) 的CPG水平更高,并可能影响宿主基因组.

科学领域:

病毒学病毒学
免疫学免疫学免疫学
基因组学就是基因组学.

背景情况:

指抗病毒蛋白 (ZAP) 参与抑制病毒CpG二核酸,使其与宿主基因组相似.
在体内证据支持ZAP在CpG抑制中的作用是有限的.
来自Cercopithecus的SIVmus和SIVmon中的异常高的CpG水平需要进行调查.

研究的目的:

调查SIVmus和SIVmon中CpG水平升高的原因.
为了确定ZAP活性是否负责这些病毒中的CpG抑制.
探索ZAP异型变异对病毒CpG含量和宿主基因组抑制的影响.

主要方法:

在不同灵长类动物物种中对ZAP异型的比较分析.
调查SIVmus对ZAP抑制的耐药性.
在病毒和宿主基因组中评估CpG水平.

主要成果:

SIVmus对ZAP抑制没有耐药性;Cercopithecus主体表达了一个不太活跃的XL ZAP异型,这是由于拼接部位突变.
在内源性前性lentiviruses中较高的CpG水平与鼠ZAPs的低活性相关.
与其他灵长类相比,的基因表现出较低的CpG抑制.

结论:

主体ZAP异型活性显著影响病毒CpG二核酸含量.
ZAP的抗病毒活性可能会影响病毒转录和宿主基因组.
在CpG抑制中ZAP的作用比以前假设的更复杂,更依赖宿主.

关键词:

抑制了CPG的抑制作用.灵长类动物的lentiviruses病毒.病毒-宿主军备竞赛指抗病毒蛋白质是一种抗病毒蛋白质.

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