对大鼠喉诱导的吞速度的影响
Satomi Kawada1, Titi Chotirungsan1,2, Charng-Rong Pan1
1Division of Dysphagia Rehabilitation, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
概括
离子,特别是在化 (KCl) 溶液中,通过刺激喉神经来增强吞反射. 特定的离子通道,即内向整流器通道 (Kir3.1和Kir6.2),对于这种由诱导的小鼠吞反应至关重要.
科学领域:
- 神经科学是一个神经科学.
- 生理学 生理学 生理学
背景情况:
- 吞反射对于营养摄入和呼吸道保护至关重要.
- 周围喉刺激可以触发吞反射.
- 离子被认为有助于吞,但底层机制尚不清楚.
研究的目的:
- 研究离子对大鼠外围 afferent反应和吞启动的影响.
- 探索参与离子诱导吞的潜在受体.
主要方法:
- 化 (KCl),蒸水 (DW) 和化 (NaCl) 的局部应用给有意识和麻醉的老鼠.
- 记录上喉神经活动的记录.
- 观察慢性大鼠的行为和吞间隔.
- 内向整流器通道 (Kir)3.1和Kir6.2.2的免疫组织化学检测
- 评估Kir3.1和Kir6.2阻塞剂对KCl诱导的吞的影响.
主要成果:
- 局部的KCl显著促进了吞反射,比DW或NaCl更多,其影响取决于离子度.
- KCl的应用导致了最突出的离子诱导的上喉神经反应,特别是在早期.
- 在慢性老鼠中,与KCl相关的显示了最短的吞间隔.
- 检测到Kir3.1和Kir6.2通道在节点关节和声 (74.1%和75.3%的表达,分别).
- 阻断Kir3.1和Kir6.2的药物显著降低了KCl诱导的吞的数量.
结论:
- 离子通过KCl溶液有效诱导喉神经反应和吞反射.
- 内向整流器通道Kir3.1和Kir6.2在中介离子诱导的小鼠吞中起着重要作用.
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