TENS改善CFL损伤的老鼠,并调节肠道微生物群
Min Miao1,2, Tong Ma1,2, Ran Chen3
1Pharmaceutical College and Key Laboratory of Pharmacology for Natural Products of Yunnan Province, Kunming Medical University, Kunming, Yunnan, P.R. China.
PloS one
|April 3, 2025
概括
通过皮肤电神经刺激 (TENS) 通过调节肠道微生物群,改善了大鼠的筋带 (CFL) 损伤. TENS可以减少炎症和致病细菌,同时增加有益的益生菌,为CFL损伤提供潜在的新疗法.
科学领域:
- 生物医学工程 生物医学工程
- 微生物学 微生物学
- 整形外科 整形外科 整形外科
背景情况:
- 脚扭伤,特别是关节带 (CFL) 损伤很常见,可能导致慢性问题.
- 目前对CFL损伤的治疗方法通常集中在物理治疗和疼痛管理上,解决潜在的炎症过程的选择有限.
- 肠道微生物群在全身炎症和愈合中的作用是一个新兴的研究领域.
研究的目的:
- 在CFL损伤的小鼠模型中研究通过皮肤电神经刺激 (TENS) 的治疗机制.
- 为了确定TENS是否可以调节CFL受伤的老鼠的肠道微生物群组成和功能.
- 探索TENS诱导的肠道微生物群变化和对CFL损伤的抗炎作用之间的关系.
主要方法:
- 建立了CFL损伤的老鼠模型,并用TENS治疗了1,2,3周.
- 通过测量运动能力和IL-1β/NF-κB/IL-17信号通路的表达来评估CFL损伤的改善.
- 使用16S rDNA测序分析了肠道微生物组成,并进行了功能分析.
主要成果:
- TENS治疗显著改善了CFL受伤老鼠的运动能力.
- TENS降低了IL-1β和NF-κB的表达,表明炎症信号通路的下调.
- 16S rDNA测序显示,TENS调节了肠道微生物群,减少了致病细菌 (Ruminococcus, Dubosiella) 和增加了有益的益生菌 (Bacteroides, Lactobacillus),这与减少炎症相关.
结论:
- 通过降低IL-1β/NF-κB信号调节和调节肠道微生物群,TENS有效地改善了大鼠的CFL损伤.
- TENS的抗炎作用部分由有益的肠道细菌介导,这表明肠道健康和带愈合之间存在联系.
- 通过TENS等干预措施准肠道微生物群,为CFL损伤提供了一个有希望的治疗策略.
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