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非线性声板显微镜:在毛细血管和细胞尺度上成像不透明的器官
Baptiste Heiles1, Flora Nelissen2, Rick Waasdorp1
1Department of Imaging Physics, Delft University of Technology, Delft, Netherlands.
Science (New York, N.Y.)
|April 3, 2025
概括
非线性声板显微镜能够在大量组织中快速体积成像生物功能. 这种先进的技术克服了光片显微镜的局限性,
科学领域:
- 生物医学成像
- 声学显微镜
- 分子成像
背景情况:
- 光板光显微镜在厚组织中受到光散射和光漂白的限制.
- 在体内研究动态细胞过程通常需要超出光线透深度的成像.
研究的目的:
- 开发一种新的成像方法,以快速和体积可视化生物功能的厘米尺度.
- 克服光学显微镜在分散和不透明的生物组织中的局限性.
主要方法:
- 使用非衍射超声波束与交叉振幅调制序列.
- 使用非线性声学报告器,包括基因编码的气囊和血管内微泡.
- 开发用于加速成像的非线性声板显微镜.
主要成果:
- 在立方厘米尺度上实现瘤基因表达体积成像.
- 使用微泡对比剂实现脑毛细血管网络的局部显微镜.
- 显示成像速度的64倍,成像体积的35倍,分辨率的4倍.
结论:
- 非线性声板显微镜为深层组织生物成像提供了显著的进步.
- 这项技术能够以前所未有的规模进行高速的体积功能成像.
- 这种方法扩大了研究体内复杂生物过程的可能性.
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