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通过纳米粒子辅助ICP-MS和电化学多式分析快速和灵敏地检测食源性病原体

Yujie Zhou¹, Lei Li¹, Zhihui Tang²

¹National Engineering Research Center for Biomaterials & College of Biomedical Engineering, Sichuan University, Chengdu, Sichuan 610065, China.

|April 3, 2025

概括

一种新方法使用磁纳米粒子和贵金属标签来快速,灵敏地检测食物传播的病原体,如沙门氏菌,氏菌和氏菌. 这种双感应技术通过同时准确识别多种细菌来确保食品安全.

科学领域:

食品科学食品科学食品科学
分析化学分析化学
微生物学微生物学

背景情况:

食物传播疾病对全球健康构成重大风险.
准确和快速检测病原体对于食品安全至关重要.

研究的目的:

开发一种新,快速,特定和敏感的方法,同时检测多种食源性病原体.
整合磁分离和贵金属纳米粒子标签,以提高病原体检测.

主要方法:

开发了一种三明治杂交技术,利用磁纳米粒子 (MNP) 和贵金属纳米粒子探针.
病原体DNA与探针形成复合体,使磁性分离成为可能.
诱导合等离子体质谱法 (ICP-MS) 和电化学微分脉冲电压测量用于双检测.

主要成果:

这种方法可以同时检测出沙门氏菌 Typhimurium,Vibrio parahaemolyticus 和 Shigella sonnei.
证明了广泛的检测范围 (10^1到10^10副本·mL^-1) 和低的检测极限 (1副本·mL^-1).
该技术在真实食品样本中成功应用,证实了其实用性.

结论:

开发的双传感技术为快速,敏感和特定检测食品传播病原体提供了强大的工具.
这种方法在改善食品安全监测和公共卫生保护方面具有重大潜力.

关键词:

电化学分析电化学分析通过食物传播的病原体对ICP-MS的分析贵金属纳米粒子是贵金属的纳米粒子.这是一种三明治杂交.

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