麦蛋白分离物的凝潜力:提取方法的影响,凝条件和酶修饰的作用
Miikka Laitinen1, Tiina Kokkonen1, Xin Huang1
1Department of Food and Nutrition, University of Helsinki, Agnes Sjöbergin katu 2, 00790 Helsinki, Finland.
Food chemistry
|April 3, 2025
概括
改善食品的麦蛋白分离物 (OPI) 功能需要优化提取和修改. 酶去化增强了溶解性和改善了凝,克服了水解和提取pH的挑战.
科学领域:
- 食品科学 食品科学 食品科学
- 蛋白质化学 蛋白质化学
- 生物技术是生物技术.
背景情况:
- 麦蛋白分离物 (OPI) 的技术功能较差,限制了其在食品中的使用.
- 了解影响OPI凝的因素对于开发新型食品应用至关重要.
研究的目的:
- 为了研究提取方法,凝条件 (pH,NaCl) 和酶修饰对麦蛋白分离物的热诱导凝的影响.
- 提高OPI的技术功能,以改善食品应用.
主要方法:
- 麦蛋白分离物 (OPI) 经过不同的提取pH值 (8和~10).
- 进行了酶修饰,包括部分水解 (酸盐,胺,帕帕因) 和除化 (蛋白质谷氨酸酶).
- 在不同的pH值和NaCl度下评估了凝性质,并通过rheological测量 (G',tan δ) 描述了凝强度和弹性.
主要成果:
- OPI凝对pH和NaCl度高度敏感;在pH ~ 10下提取与pH 8相比降低了凝特性.
- 部分水解增加了OPI的溶解度,但影响了凝,导致协同作用.
- 用蛋白质谷氨酸酶进行酶去化,在pH7下达到87%的溶解度,并产生柔软,弹性的凝.
结论:
- 提取条件和酶修饰显著影响麦蛋白分离物的技术功能.
- 酶去化为改善OPI溶解性和凝性质的食品应用提供了一个有前途的策略.
- 需要进一步优化麦蛋白加工,以克服目前食品配方的局限性.
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