塑料化学物质通过体外腺1受体破坏分子昼夜节律
Molly McPartland1, Felicity Ashcroft1, Martin Wagner1
1Department of Biology, Norwegian University of Science and Technology (NTNU), 7491 Trondheim, Norway.
Environment international
|April 3, 2025
概括
塑料化学物质激活腺1受体 (A1R),破坏调节睡眠-清醒周期的细胞信号. 这项研究揭示了一条新的途径,将塑料暴露与昼夜节律扰乱和潜在的非传染性疾病联系起来.
科学领域:
- 内分泌学 在内分泌学.
- 环境健康 环境健康
- 分子生物学分子生物学
背景情况:
- 腺素1受体 (A1R) 对于调节睡眠-清醒周期和昼夜节律至关重要.
- 塑料制品含有大量的化学物质,有可能破坏生理功能.
- 一些塑料化学物质是已知的A1R激动剂,但它们对细胞功能和昼夜节律的下游影响在很大程度上是未知的.
研究的目的:
- 为了调查聚氨 (PUR) 和聚乙烯 (PVC) 塑料中的化学物质是否通过A1R激动性扰乱昼夜节律.
- 为了确定A1R.的塑性化学激活的细胞效应.
- 量化塑料化学品对关键昼夜钟基因表达的影响.
主要方法:
- 从PUR和PVC塑料中提取化学品.
- 在U2OS细胞中激活A1R和抑制细胞内cAMP.
- 在4小时间隔的48小时内量化PER2和CRY2钟基因表达.
- 评估使用药理抗剂的剂量依赖性和可逆性.
主要成果:
- 来自PUR和PVC的塑料化学物质激活了U2OS细胞中的A1R,并抑制了U2OS细胞中的细胞内cAMP,与特定的A1R激动剂相当.
- 对塑料化学品的暴露导致PER2和CRY2时钟基因的振荡表达发生了9-17分钟的相位变化.
- 观察到的昼夜干扰是剂量依赖的,在A1R抑制后是可逆的.
结论:
- 塑料化学物质通过激活A1R并干扰其信号通路来破坏昼夜过程.
- 这项研究确定了一种新的机制,塑料化学物质可以影响昼夜节律.
- 塑料诱导的A1R激素可能有助于非传染性疾病的发展.
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