硫酸功能化共价有机框架@Ti3C2Tx作为有效的固相微提取叶片涂层,用于从大鼠血清样本中提取单胺神经递质
Cheng Yang1, Nian Yang1, Di Zhao1
1Henan University of Chinese Medicine, Zhengzhou 450046, PR China.
Journal of chromatography. A
|April 3, 2025
概括
一种新的硫功能化共价有机框架@Ti3C2Tx纳米复合材料被开发用于单胺神经递质 (MNT) 的固相微提取 (SPME). 这种方法在老鼠血清中提供了灵敏而准确的MNT分析.
科学领域:
- 材料科学 材料科学 材料科学
- 分析化学 分析化学
- 生物化学 生物化学
背景情况:
- 单胺神经递质 (MNT) 是神经疾病的关键生物标志物.
- 在血清等生物矩阵中精确量化MNT是具有挑战性的,因为其度低,矩阵复杂.
- 开发先进的提取技术对于敏感的MNT检测至关重要.
研究的目的:
- 合成一个硫功能化共价有机框架@Ti3C2Tx (SO3HCOF@Ti3C2Tx) 纳米复合材料用于固相微提取 (SPME).
- 开发和优化SPME高性能液体染色学 (HPLC) 方法,用于隔离和确定大鼠血清中的MNT.
- 评估开发的方法的有效性,用于分析MNTs从抑郁症模型大鼠的血清.
主要方法:
- 合成SO3HCOF@Ti3C2Tx纳米复合材料,具有混合的水友和功能性质.
- 纳米复合材料作为SPME涂层用于从老鼠血清中提取MNT的应用.
- 使用HPLC优化SPME条件和随后的分析.
- 验证该方法的线性,检测极限,矩阵效应和恢复.
主要成果:
- 这种SO3HCOF@Ti3C2Tx纳米复合材料对MNTs表现出多个吸附点和相互作用 (离子交换,结,π-π).
- 该SPME-HPLC方法显示出优异的线性 (r2 ≥0.9963),低检测极限 (0.0150.030 ng mL-1),以及最小的矩阵效应 (0.83%17.36%).
- 在大鼠血清中,MNT的高恢复率 (90.3%118.3%) 与较低的相对标准偏差 (RSDs <10.8%) 得到了实现.
结论:
- 开发的SO3HCOF@Ti3C2Tx纳米复合材料是用于MNT提取的有效SPME材料.
- SPME-HPLC方法提供了一种敏感,可靠和高效的策略,用于确定复杂生物样本中的微量MNT.
- 这种方法适用于从抑郁症模型大鼠的血清样本中分析MNT,有助于神经疾病研究.
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