绿色溶剂在液体染色学中作为移动相的潜力
1Univ. Bordeaux, Bordeaux INP, INRAE, OENO, UMR 1366, ISVV, F-33140 Villenave d'Ornon, France.
Journal of chromatography. A
|April 3, 2025
概括
像乙醇这样的绿色溶剂在逆相高性能液态染色学 (RP-HPLC) 中被广泛使用,但由于安全性和性能问题,需要替代品. 研究探索RP-HPLC的新绿色溶剂,面临UV吸收和可用性方面的挑战.
科学领域:
- 分析化学 分析化学
- 绿色化学 绿色化学
- 染色体学 染色体学 是一种染色学.
背景情况:
- 逆相高性能液态色谱 (RP-HPLC) 传统上使用有机溶剂,引发了环境和安全方面的担忧.
- 越来越多地探索绿色溶剂以减轻环境影响并提高染色学方法的安全性.
- 乙醇和是RP-HPLC中确定的绿色溶剂,但存在粘度,紫外线吸收性,挥发性和易燃性等局限性.
研究的目的:
- 审查RP-HPLC中各种绿色溶剂的应用和性能.
- 突出以乙醇作为绿色溶剂的确立使用及其相关挑战.
- 探索新兴的绿色溶剂及其解决传统选择局限性的潜力.
主要方法:
- 1990年至今发表的135篇文章的综合文献综述,重点关注RP-HPLC中的绿色溶剂.
- 在RP-HPLC中分析乙醇-水移动相,检查方法参数,如列粒子大小和紫外线检测波长.
- 对替代绿色溶剂的评估,包括乙基乳酸盐,烯碳酸盐,二甲基碳酸盐,甲基酸盐,锡林,甘油和天然深度环氧化溶剂.
主要成果:
- 乙醇是RP-HPLC中广泛采用的绿色溶剂,通常用于减少颗粒直径的柱子和低紫外线检测波长.
- 尽管乙醇普遍存在,但其高粘度,紫外线吸收性,挥发性和易燃性存在重大缺点.
- 新兴的绿色溶剂,如Cyrene和糖显示承诺,但面临挑战,包括紫外线吸收,水不混合性,高粘度和有限的HPLC级可用性.
结论:
- 乙醇仍然是RP-HPLC中常见的绿色溶剂,但其局限性需要探索更安全,更有效的替代品.
- 新的绿色溶剂为VOC问题提供了潜在的解决方案,但需要进一步开发以克服当前的性能和可用性障碍.
- 额外的研究至关重要,以促进在RP-HPLC应用中更广泛地采用先进的绿色溶剂.
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