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乙酶响应的卡特基因复合水凝微球促进细胞核的再生在椎间盘退化

Junqi Dai¹, Li Ni², Chenyang Jin²

¹Department of Orthopaedics, The First Affiliated Hospital of Soochow University, MOE Key Laboratory of Geriatric Diseases and Immunology, Suzhou Medical College, Soochow University, Suzhou, 215000, Jiangsu, China; Orthopaedic Institute, Suzhou Medical College, Soochow University, Suzhou 215007, China; Department of Orthopaedics, Taixing People's Hospital, Taixing 225400, China.

Acta biomaterialia

|April 3, 2025

概括

这项研究开发了一种新型的水凝微球 (GHKM),用于输送细胞用于核脉再生,改善退化的磁盘中的细胞存活和功能. GHKM通过模仿细胞外基质并释放治疗性卡尔托基因因,有效地促进椎间盘退化修复.

科学领域:

生物材料科学生物材料科学
再生医学是一种再生医学.
组织工程是组织工程.

背景情况:

椎间盘退化 (IVDD) 是一个重要的健康问题.
退化的磁盘的敌对微环境阻碍了细胞移植疗法.
目前的策略面临的挑战是细胞存活和细胞集成用于核脉 (NP) 再生.

研究的目的:

开发一种仿生,对酶有反应性的复合水凝微球 (GHKM),用于IVDD中增强细胞传递.
为了克服NP再生退化的磁盘微环境的局限性.
评估GHKM在促进NP再生中的疗效,无论是体外还是体内.

主要方法:

使用凝甲酸盐 (GelMA) 和HAMA-KGN结合物制造GHKM.
在实验室中评估GHKM生物相容性,细胞粘附,增殖和ECM合成的细胞核细胞 (NPCs).
转录组测序以分析分子机制,包括抗氧化途径和NRF2激活.
在鼠尾核切除模型中体内植入载有NPC的GHKM.

主要成果:

GHKM模仿NP细胞外基质,支持细胞增殖和适应不利条件.
从GHKM中释放以乙酶反应的卡特基因 (KGN) 促进ECM合成,并减少氧化应激.
转录组分析显示了抗氧化途径的丰富和NRF2激活.
在体内研究表明保存的圆盘高度,结构和功能,证实了NP再生.

结论:

在IVDD治疗中,GHKM是用于细胞输送的有希望的生物材料.
复合水凝微球有效地应对退化的磁盘微环境的挑战.
GHKM为NP再生提供了一种协同方法,显示了临床翻译的潜力.

关键词:

对乙酶有反应性的水凝微球是水凝的微球.椎间盘退化椎间盘退化卡托基因在纸张中存在.再生再生再生的过程

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