在肥胖的情况下,Formin INF2补充剂减轻了基于细胞骨的线粒体缺陷,以改善卵细胞质量
Hao-Lin Zhang1, Zhen-Nan Pan1, Jia-Qian Ju1
1College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
Free radical biology & medicine
|April 3, 2025
概括
肥胖会通过影响卵细胞质量来损害女性的生育能力. 这项研究表明,肥胖卵细胞中减少的反转型二甲 (INF2) 破坏了actin聚合和线粒体功能,导致成熟缺陷.
科学领域:
- 生殖生物学 生殖生物学
- 细胞生物学 细胞生物学
- 分子医学是分子医学.
背景情况:
- 肥胖是女性生殖障碍的重要原因,导致卵细胞中介成熟缺陷和不孕症.
- 肥胖卵细胞功能障碍背后的分子机制在很大程度上仍未被描述.
研究的目的:
- 在肥胖的背景下,研究逆转型二甲 (INF2) 在卵细胞质量和中性成熟中的作用.
- 阐明INF2影响卵细胞成熟的分子机制.
主要方法:
- 从肥胖和对照小鼠的卵细胞中对INF2表达的定量分析.
- 评估极体挤出,螺旋转移和线粒体的分布/功能.
- 调查INF2与actin,DRP1,HDAC6和氨酸乙化的相互作用.
- 使用外源INF2 mRNA补充的救援实验.
主要成果:
- 在肥胖小鼠的卵细胞中,INF2表达减少.
- 缺少INF2导致极体挤出失败,极体大,并扰乱了线粒体的分布和功能.
- 通过对氨酸乙化的影响,INF2枯竭损害了actin聚合,螺旋转移和线粒体运输.
- 补充INF2mRNA可以挽救肥胖卵细胞中中性成熟缺陷.
结论:
- 在哺乳动物卵细胞成熟过程中,INF2发挥着至关重要的作用,通过调节线粒体功能和线粒体组织必不可少的活性蛋白聚合和蛋白乙化.
- 鉴于INF2缺乏症被认为是肥胖引起的卵细胞缺陷的潜在分子原因,影响女性生育能力.
相关概念视频
02:07Oogenesis
63.2K
In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...
63.2K
02:02Meiosis II
40.9K
Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
40.9K