格雷·海根 3.6-like 通过负调节葡萄藤中基本螺旋环-螺旋环转录因子106的表达,促进了氨酸的积累
Shixiong Lu1, Juanbo Yang1, Guangling Shi1
1College of Horticulture, Gansu Agricultural University, Lanzhou 730070, PR China.
International journal of biological macromolecules
|April 3, 2025
概括
一个葡萄藤基因,类似于VaGH3.6,促进了安东素和黄素的积累. 它与VabHLH106相互作用,这是一个负调节器,揭示了葡萄中安东氨酸合成的新反循环.
科学领域:
- 植物分子生物学 植物分子生物学
- 生物化学 生物化学
- 遗传学 是一个遗传学.
背景情况:
- 安索是重要的植物颜料,来源于黄类前体.
- 格雷·海根3 (GH3) 基因家族在氨酸生物合成中的作用在很大程度上尚未被探索.
- 了解监管机制是提高葡萄品质的关键.
研究的目的:
- 为了研究VaGH3.6类基因在葡萄藤中安东素生产中的功能.
- 阐明VaGH3.6-like和VabHLH106转录因子之间的调控关系.
- 为了揭示控制葡萄中安东素积累的分子机制.
主要方法:
- 在葡萄藤和果皮的基因鉴定和过度表达.
- 在不同的光照条件下分析安托西亚尼和黄类积累.
- 用于转录因子查的RNA测序 (RNA-seq).
- 酵母两杂交 (Y2H),酵母一杂交 (Y1H),双路西法酶报告器 (DLRTM) 和电泳运动移位试验 (EMSA) 进行分子相互作用.
主要成果:
- 过度表达的VaGH3.6-like增加了安东素和黄素的积累,并降低了英多尔-3-酸 (IAA) 水平.
- 确定了一种负调节剂VabHLH106,该调节剂直接与VaGH3.6类促进子结合.
- 与VaGH3.6类似的过度表达启动了负反循环,缓解了下游安托素合成基因上的VabHLH106抑制.
结论:
- 类似于VaGH3.6的葡萄在葡萄中促进安托素和黄类生物合成方面发挥着至关重要的作用.
- 类似VaGH3.6和VabHLH106的相互作用形成了一个调节网络,影响了安托西亚尼的积累.
- 这项研究为控制葡萄安托西亚尼合成的分子机制提供了新的见解.
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