具有编程药物释放的DNA支架框架的自然杀手细胞,用于化疗采用细胞治疗
Shiyi Bi1, Jieyu Shen1, Yu Zhu1
1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
概括
这项研究提出了一种新的DNA支架系统,用于联合化疗-采用细胞疗法. 它保护NK细胞,同时允许定时的药物释放和细胞激活,以加强癌症治疗.
科学领域:
- 生物医学工程 生物医学工程
- 纳米技术纳米技术
- 免疫治疗是一种免疫疗法.
背景情况:
- 有效的联合化疗 - 收养细胞疗法需要优化药物输送和细胞输送时间.
- 收养免疫细胞是潜在的药物载体,但可以受到化学毒性有效载荷的影响.
- 目前的方法努力平衡药物输送与免疫细胞功能.
研究的目的:
- 开发一种用于联合化疗 - 收养细胞治疗的新型输送系统.
- 为了保护自然杀手 (NK) 细胞免受化疗有效载荷的影响.
- 为了实现时间编程的药物释放和NK细胞激活,以增强治疗效果和最小化副作用.
主要方法:
- 制造一个包装NK细胞的多孔DNA支架系统.
- 将IL-21纳米颗粒 (通过GSH可切割链接器) 和化疗有效载荷 (通过ROS可切割链接器装载DOX/维拉帕米尔PLGA纳米颗粒) 附着在DNA支架外部.
- 利用瘤微环境刺激 (ROS和还原环境) 进行连续的药物释放和NK细胞激活.
主要成果:
- 这种DNA支架保护了NK细胞的功能,同时保持了瘤定位能力.
- 特定于瘤的ROS触发了化疗纳米粒子 (PLGA药物NP) 的释放.
- 随后的还原性环境导致DNA支架脱离,释放NK细胞和IL-21用于协同治疗.
结论:
- 开发的DNA支架系统通过脱药物管理和细胞输送,使有效的化疗采用细胞疗法成为可能.
- 这种方法通过保护免疫细胞来最大限度地减少副作用,并允许定时释放药物和细胞激活.
- 该系统通过协同化学疗法和免疫疗法证明了增强的治疗效率.
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