相关实验视频

Updated: May 17, 2025

Preparation of Myeloid Derived Suppressor Cells MDSC from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting AutoMACS

Preparation of Myeloid Derived Suppressor Cells MDSC from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting AutoMACS

Published on: June 18, 2012

在多发性骨髓瘤中PD-L1表达骨髓瘤衍生抑制细胞

Laura G Rico¹, Roser Salvia¹, Jolene A Bradford²

¹Functional Cytomics Lab, Germans Trias i Pujol Research Institute (IGTP), ICO-Hospital Germans Trias i Pujol, Universitat Autònoma de Barcelona, Badalona, Barcelona, Spain; Department of Cellular Biology, Physiology and Immunology, Autonomous University of Barcelona (UAB), Cerdanyola del Vallès, Spain.

Methods in cell biology

|April 3, 2025

概括

一种新的流细胞计测试检测了多发性骨髓瘤中髓质衍生抑制细胞上的编程细胞死亡1 (PD-L1) 的结构变化. 这种方法有助于对患者进行分类,并研究免疫疗法药物相互作用.

科学领域:

免疫学免疫学免疫学
在瘤学瘤学.
生物技术是生物技术.

背景情况:

编程细胞死亡蛋白1/编程细胞死亡蛋白连接体1 (PD-1/PD-L1) 轴是癌症免疫疗法的关键目标.
在多发性骨髓瘤 (MM) 中,PD-L1 过度表达,但在骨髓系衍生抑制细胞 (MDSC) 上对抗体识别具有构造性挑战.

研究的目的:

开发一种流细胞测量试验,用于检测MM中MDSC的PD-L1形状变化.
为了根据PD-L1表达来进行患者分类,并研究免疫疗法相互作用.

主要方法:

开发一种流细胞计查试验,采用最小的样本操纵,以保持PD-L1结构.
从MM患者的MDSC中评估PD-L1水平,有和没有刺激 (PMA).
对PD-L1表达的动态分析和对durvalumab的竞争性结合实验.

主要成果:

该试验在刺激后成功检测了MDSCs的PD-L1的构造变化.
可以根据不同的PD-L1检测后刺激来分类MM患者.
建立了评估PD-L1动力学,亚细胞局部化和药物相互作用的协议.

结论:

开发的流细胞测量试验对于评估MDSCs中的PD-L1构造变化是有效的.
这种方法为MM患者分层和评估PD-1/PD-L1向治疗提供了一种新方法.
该测试平台在研究其他蛋白质的结构变化方面具有潜在的应用.

关键词:

检查点抑制剂检查点抑制剂流动细胞计量流动细胞计量免疫治疗是一种免疫疗法.多发性骨髓瘤是一种多发性骨髓瘤.在 PDL1 中,PDL1 是 PDL1.

更多相关视频

Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells

Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells

Published on: January 29, 2019

Author Spotlight: Magnetic Fluorescent Bead-Based Dual-Reporter Flow Analysis of PDL1-Vaxx Peptide Vaccine-Induced Antibody Blockade of the PD-1/PD-L1 Interaction

Author Spotlight: Magnetic Fluorescent Bead-Based Dual-Reporter Flow Analysis of PDL1-Vaxx Peptide Vaccine-Induced Antibody Blockade of the PD-1/PD-L1 Interaction

Published on: July 7, 2023

相关概念视频

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...