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相关概念视频

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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多复合细胞计学用于单细胞化学生物学.

Henry A M Schares1, Madeline J Hayes2, Joseph A Balsamo3

  • 1Department of Chemistry, Vanderbilt University, Nashville, TN, United States; Vanderbilt Institute of Chemical Biology, Nashville, TN, United States; Vanderbilt Chemical and Physical Biology Program, Vanderbilt University, Nashville, TN, United States.

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该协议将化学和生物学联系起来,用于使用流细胞计进行化学生物学发现. 它使初学者能够选小分子和细胞类型的功能读数.

关键词:
化学生物学是化学生物学.复合选 复合选 复合选 复合选 复合选流动细胞计量流动细胞计量光细胞条形码编码代谢学 代谢学 代谢学自然产品的发现自然产品的发现.一个单细胞的单细胞.培训协议 培训协议

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科学领域:

  • 化学生物学 化学生物学
  • 细胞生物学 细胞生物学
  • 翻译研究是翻译研究.

背景情况:

  • 流细胞计提供深度细胞定量和高吞吐量,非常适合转化研究.
  • 化学生物学中的不足利用源于化学家和细胞生物学家之间的专业知识差距.
  • 弥合这一差距需要新手研究人员可访问的协议.

研究的目的:

  • 为化学生物学发现提供详细的化学和生物学桥梁协议.
  • 培训初学者研究人员进行功能性细胞基细胞计测试.
  • 鼓励在发现和转化研究中使用流细胞计.

主要方法:

  • 介绍了一种适应小分子输入 (微生物提取物,纯化合物) 和多种细胞类型 (初级人类,小鼠,癌症细胞系) 的协议.
  • 详细介绍细胞测量读数用于细胞功能,包括DNA损伤反应,生长,细胞亡,细胞周期和活力.
  • 包含96井板格式,光细胞条形码和debarcodeR算法,用于简化分析.

主要成果:

  • 该协议可以选择输入,目标细胞和细胞计读出模块.
  • 对于各种功能性读取,如细胞身份,分化和损伤机制,表现出适应能力.
  • 快速为数百万个细胞提供细胞特征的深度量化.

结论:

  • 该协议使化学生物学家能够将功能性基于细胞的细胞计整合到发现管道中.
  • 它为更广泛的研究社区民主使用先进的流细胞计技术.
  • 旨在通过可访问,高通量选方法,促进化学生物学领域的创新.