相关实验视频

Updated: May 17, 2025

Automated Multiplex Immunofluorescence Panel for Immuno-oncology Studies on Formalin-fixed Carcinoma Tissue Specimens

Automated Multiplex Immunofluorescence Panel for Immuno-oncology Studies on Formalin-fixed Carcinoma Tissue Specimens

Published on: January 21, 2019

自动化细胞计量在免疫瘤学的贡献

Andrea Sbrana¹, Giuliano Mazzini², Giuditta Comolli³

¹Department of Surgical, Medical and Molecular Pathology and Critical Care Area, University of Pisa, Pisa, Italy; Service of Pneumo-Oncology, Unit of Pneumology, Pisa, Italy.

Methods in cell biology

|April 3, 2025

概括

了解对癌症免疫疗法的耐药性至关重要. 像流细胞计 (FCM) 和飞行时间细胞计 (CyTOF) 这样的先进技术可以监测免疫细胞,但对于临床使用需要标准化.

科学领域:

免疫学免疫学免疫学
在瘤学瘤学.
生物技术是生物技术.

背景情况:

癌症免疫疗法提供了显著的生存益处,但耐药性仍然是一个挑战.
了解免疫机制是克服治疗耐药性的关键.
目前的临床实践缺乏可靠的方法来监测这些机制.

研究的目的:

探索先进的细胞计量技术在癌症免疫治疗中监测免疫机制的潜力.
确定这些方法如何阐明免疫细胞功能和瘤微环境中的相互作用.
评估使用自动化细胞计量用于药物向参与分析的可行性.

主要方法:

使用流细胞计 (FCM) 和飞行时间细胞计 (CyTOF) 进行免疫细胞的多参数分析.
分析蛋白质表达模式,以了解免疫细胞功能,包括T细胞.
调查药物标相互作用研究的受体占用率.

主要成果:

FCM和CyTOF能够同时分析多个蛋白质表达,有助于理解免疫细胞群和它们的相互作用.
这些技术可以通过受体占用来评估药物向相互作用.
局限性包括光谱重叠,细胞数量低,需要活跃的细胞,缺乏标准化的协议.

结论:

先进的细胞计量对监测免疫机制和了解癌症免疫疗法耐药性的有希望.
标准化和克服技术限制对于临床翻译至关重要.
目前正在进行的试验旨在确定这些强大的技术的临床适用性.

关键词:

临床瘤学临床瘤学诊断和治疗诊断和治疗流动细胞计量流动细胞计量免疫瘤学免疫瘤学免疫治疗是一种免疫疗法.固体瘤是一种固体瘤.

更多相关视频

A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood

A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood

Published on: February 8, 2016

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Published on: March 25, 2020

相关概念视频

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

Tumor Immunotherapy

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...