探索新的自然产品,利用未开发的二次代谢途径在actinomycetes
1Department of Life Science, Faculty of Science, Gakushuin University, 1-5-1 Mejiro, Toshima-ku, Tokyo, 171-8588, Japan. shotaro.hoshino@gakushuin.ac.jp.
Journal of natural medicines
|April 3, 2025
概括
从actinomycetes中发现新的生物活性化合物是具有挑战性的. 这项研究使用共同培养激活了actinobacteria中的静态基因集群,产生了16种新的天然产品,并揭示了独特的代谢.
科学领域:
- 微生物学 微生物学
- 自然产品化学 自然产品化学
- 基因组学就是基因组学.
背景情况:
- 动菌体是生物活性二次代谢产物的丰富来源.
- 发现新天然产品的传统方法越来越困难.
- 基因组数据揭示了许多未开发的二次代谢物生物合成基因集群 (SM-BGCs) 在actinobacteria.
研究的目的:
- 探索尚未开发的SM-BGC的潜力,以发现新型的动氨酸细菌天然产品.
- 为了激活沉默的SM-BGC,并识别新的二次代谢产物.
- 为了调查被忽视的与有关的二次新陈代谢在actinomycetes.
主要方法:
- 与含有酸的细菌 (MACB) 共同培养actinomycetes以激活沉默的SM-BGCs.
- 二次代谢产物的鉴定和表征.
- 生物合成途径的解,包括代谢.
主要成果:
- 沉默的SM-BGCs的激活导致了20种活性细菌的二次代谢物的鉴定,其中包括16种新的化合物.
- 发现了一种新的有机天然产品.
- 阐明一种独特的生物合成策略,独立于依赖S-adenosylmethionine (SAM) 的酶.
结论:
- 与MACB共同培养是一种有效的策略,用于激活沉默的SM-BGC,并发现新的动态细菌天然产品.
- 与相关的二次新陈代谢代表了actinomycetes的新和未经探索的领域.
- 鉴定的有机天然产品及其生物合成途径为微生物代谢提供了新的见解.
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