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Updated: May 16, 2025

Sample Preparation for Mass Cytometry Analysis

Sample Preparation for Mass Cytometry Analysis

Published on: April 29, 2017

在CyTOF中进行样本复合:优化单细胞蛋白质基因分析的途径.

Muharrem Muftuoglu¹, Michael Andreeff¹

¹Section of Molecular Hematology and Therapy, Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX, 77030, USA.

|April 4, 2025

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通过飞行时间 (CyTOF) 的细胞计测试中样本复合增加了蛋白质组分析的深度. 新的化剂改进了金属同位素标记,用于同时进行多样本分析,提高了数据准确性和发现潜力.

科学领域:

生物技术是生物技术.
蛋白质组学是指蛋白质组学.
分析化学分析化学

背景情况:

单细胞蛋白质组分析对于理解细胞异质性至关重要.
飞行时间细胞计 (CyTOF) 是用于高维单细胞分析的强大技术.
样本吞吐量和批量效应的限制可能会阻碍深入的蛋白质组学研究.

研究的目的:

使用CyTOF提高单细胞蛋白质组分析的深度和效率.
引入和评估用于金属同位素标记的新型聚合物基化剂.
在CyTOF实验中展示样品多重复合的好处.

主要方法:

开发用于金属同位素结合的新型聚合物基剂.
在 CyTOF 工作流程中实施样本复杂化策略.
多样化与单样样本 CyTOF 实验的比较分析.

主要成果:

样本复合显著增加了单细胞蛋白质组分析的深度.
新型合剂能够对多个样本进行可靠的标记和同时分析.
多重复合最小化了批量效应和保存的样本材料.

结论:

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Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Published on: December 9, 2022

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

Published on: May 1, 2017

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Last Updated: May 16, 2025

Sample Preparation for Mass Cytometry Analysis

Sample Preparation for Mass Cytometry Analysis

Published on: April 29, 2017

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Published on: December 9, 2022

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

Published on: May 1, 2017

样本复杂化是推动基于CyTOF的单细胞蛋白质组研究的关键策略.
开发的化剂扩大了金属同位素在多样本分析中的实用性.
这些进步通过简化,经济高效的实验,促进了更准确的生物发现.