人类诱导的多能干细胞被瘤性疹病毒感染
Jiaojiao Fan1, Zhen Lin2, Huiliang Zhang3
1Department of Pathology, Winthrop P. Rockefeller Cancer Institute, University of Arkansas for Medical Sciences, Little Rock, AR, United States.
Frontiers in cellular and infection microbiology
|April 4, 2025
概括
卡波西肉瘤相关的疹病毒 (KSHV) 感染人类诱导的多能干细胞 (hiPSC),导致细胞亡和改变基因表达. 这些发现突出了hiPSC作为研究KSHV病变的模型.
科学领域:
- 病毒学 病毒学
- 干细胞生物学 干细胞生物学
- 在瘤学瘤学.
背景情况:
- 卡波西肉瘤相关的疹病毒 (KSHV) 是一种与卡波西肉瘤 (KS) 和原发性输液淋巴瘤 (PEL) 相关的人类瘤病毒.
- 人类诱导多能干细胞 (hiPSC) 是从体细胞生成的,具有与胚胎干细胞类似的特性.
- hiPSCs对KSHV感染的宽容性以及随后对细胞基因概况的影响在很大程度上仍未被探索.
研究的目的:
- 为了调查hiPSC是否易受KSHV感染.
- 评估KSHV感染对hiPSC活力和亡的影响.
- 为了分析KSHV感染的hiPSCs的全球细胞基因表达变化.
主要方法:
- 确立了 hiPSCs 的 KSHV 感染.
- 使用流式细胞计量来评估hiPSC活力和感染后的亡.
- 使用Illumina RNA测序,对受感染和诱导细胞进行了全面的转录组分析.
主要成果:
- 在hiPSC中,KSHV建立了潜在的感染,并有可能发生Lytic的活性化和传染性病毒的产生.
- KSHV感染通过诱导亡来抑制hiPSC的生长.
- 在KSHV感染的hiPSC和在Lytic诱导过程中观察到全球细胞基因表达的显著变化.
结论:
- hiPSCs对KSHV感染具有宽容性,支持潜伏和溶性病毒生命周期.
- KSHV感染显著影响hiPSC活力和基因表达特征.
- hiPSCs 作为一种有价值的模型,用于研究 KSHV 病原体及其对干细胞分化的影响.
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